Pathotype grouping Xanthomonas oryzae pv. oryzae isolate from North Sumatra, Indonesia using local differential rice plants Available to Purchase

Bacterial leaf blight is one of the main diseases in rice in Indonesia and Asian countries caused by the bacterium Xanthomonas oryzae pv. oryzae (Xoo). Xoo bacteria can cause crop failure so that it can cause economic losses. This study aims to analyze the grouping of pathotypes of Xanthomonas oryzae pv. oryzae isolates from North Sumatra using local differential rice plants. The research was carried out in several stages, namely isolation and characterization of Xoo and its inoculation on 5 local differential varieties (Kencana, PB5, Tetep, Kuntulan, and Jawa 14). The results of isolation and characterization of Xoo in rice plants were gram negative, catalase positive, positive oxidase, negative growth at 0.1% TZC, positive growth on YDC medium, resistance to 0.001% Cu(NO3)2, negative starch hydrolysis, and growth in SPA medium showed yellow, round, smooth, convex, and slimy characters. The results of grouping the pathotypes of Xoo bacteria isolates from North Sumatra were grouped into pathotypes IV (70%), X (10%), XI (10%), and N (10%). Xoo isolates from North Sumatra tend to lead to malignant strains. The results of the study are expected to provide important information on the X. oryzae p.v oryzae pathotype in North Sumatra to be used in determining the development of future agricultural strategies.