The Technological pedagogical content knowledge (TPACK) framework describes the kinds of knowledge required by teachers for the successful integration of technology in teaching. It suggests that teachers need to know about the intersections of technology, pedagogy, and content. Specifically, how these areas of knowledge interact and influence one another in unique and specific contexts. This study aims to analyze preservice Biology students knowledge about TPCK. About 203 Preservice Biology students involved in this descriptive study. By administered 50 TPCK items then analyzed descriptively and item analysis individually, the result showed the average percentage of their technological knowledge is 63.53%, pedagogical knowledge is 61.78%, and content knowledge is 74.15%. Technological knowledge includes their knowledge about blended learning and IT-based media. Pedagogical knowledge includes learning theories, learning objectives, evaluation, learning strategies and approaches, learning resources, and lesson plan. Content knowledge includes biology practicum, scientific methods, virus, bacteria, moss, fern, vertebrate, gymnosperm, angiosperm, cell, human organ system, and metabolism. The study implies the importance of advancing and reinforcing some pedagogical course such as teaching learning strategies, curriculum analysis, evaluation of biology teaching learning to build better preservice biology students acquisition of TPCK.

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