Design of experiment (DoE) in reducing sugar optimization to produce third-generation bioethanol from Chlorella pyrenoidosa: Central composite design vs Box-Behnken design Available to Purchase

With the growing research in bioethanol production focusing on green algae, studying the parameters during the hydrolysis that will influence the quality of the end product is important. In this case, the design of experiment (DoE) serves as a critical start especially in terms of considering the overall cost. This study compares the design DoE based on Central Composite Design (CCD) and Box-Behnken Design (BBD) in the hydrolysis stage in producing reducing sugar from Chlorella pyrenoidosa. With four employed parameters (i.e., feedstock loading, NaOH concentration, hydrolysis temperature, and hydrolysis time), using response surface methodology (RSM), 30 experimental runs were generated from CCD and 29 from BBD. As the employed factors between the two DoEs were examined, BBD was more preferable in meeting lower cost constrained.