The negative impact caused by free radicals is very detrimental to the body, therefore antioxidants are needed that can protect the negative effects that arise and are relatively easy to obtain, one of which is the use of essential oil from basil (Ocimum sanctum) leaves. This study aims to measure the natural antioxidant activity of basil essential oil. In this study, basil leaves were distilled using the Stahl distillation method. Basil essential oil was then tested by Thin Layer Chromatography (TLC), with the mobile phase in the form of a mixture of chloroform and benzene (1:1). The results showed purple spots on the TLC GF 254 plate when observed using UV 254 light with an Rf value of 0.73 which was proven to contain terpene compounds. The antioxidant activity test of basil essential oil was carried out using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, which was measured using a UV-Vis spectrophotometer at max 517 nm. The IC50 value of the sample is 71.22 ppm and is classified as active as an antioxidant.
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27 April 2023
PROCEEDINGS OF THE SYMPOSIUM ON ADVANCE OF SUSTAINABLE ENGINEERING 2021 (SIMASE 2021): Post Covid-19 Pandemic: Challenges and Opportunities in Environment, Science, and Engineering Research
18–19 August 2021
Bandung, Indonesia
Research Article|
April 27 2023
Antioxidant activity of essential oil from basil (Ocimum sanctum L.)
Nunung Kurniasih;
Nunung Kurniasih
a)
Chemistry Department, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung
, Bandung, Indonesia
a)Corresponding author: [email protected]
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Muhamad Latief;
Muhamad Latief
Chemistry Department, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung
, Bandung, Indonesia
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Asep Supriadin;
Asep Supriadin
Chemistry Department, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung
, Bandung, Indonesia
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Assyifa Junitasari
Assyifa Junitasari
b)
Chemistry Department, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung
, Bandung, Indonesia
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a)Corresponding author: [email protected]
AIP Conf. Proc. 2646, 030008 (2023)
Citation
Nunung Kurniasih, Muhamad Latief, Asep Supriadin, Assyifa Junitasari; Antioxidant activity of essential oil from basil (Ocimum sanctum L.). AIP Conf. Proc. 27 April 2023; 2646 (1): 030008. https://doi.org/10.1063/5.0113911
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