The negative impact caused by free radicals is very detrimental to the body, therefore antioxidants are needed that can protect the negative effects that arise and are relatively easy to obtain, one of which is the use of essential oil from basil (Ocimum sanctum) leaves. This study aims to measure the natural antioxidant activity of basil essential oil. In this study, basil leaves were distilled using the Stahl distillation method. Basil essential oil was then tested by Thin Layer Chromatography (TLC), with the mobile phase in the form of a mixture of chloroform and benzene (1:1). The results showed purple spots on the TLC GF 254 plate when observed using UV 254 light with an Rf value of 0.73 which was proven to contain terpene compounds. The antioxidant activity test of basil essential oil was carried out using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, which was measured using a UV-Vis spectrophotometer at max 517 nm. The IC50 value of the sample is 71.22 ppm and is classified as active as an antioxidant.

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