This study studied the comparison of electrical potential, as well as the decrease in Chemical Oxygen Demand (COD) and Biological Oxygen Demand (BOD) Microbial Fuel Cell (MFC) using Ambon banana peel waste (MFC-KP) and tempeh waste (MFC-LT) as substrates. The MFC system used is dual chamber MFC with KMnO4 as catholyte and bentonite earthenware as membrane. Measurement of current and voltage every 2 hours during 48 hours. COD and BOD measurements were carried out before and after the waste was used in the MFC system. The measurement results show that the potential electricity of MFC-KP is higher than that of MFC-LT based on the parameters of current, voltage, and power density. MFC-KP has the highest current of 3.50 mA with an average of 1.75 mA, the highest voltage of 0.98 V with an average of 0.54 V, the highest power density of 111 mW/cm2 with an average of 43.05 mW /cm2. The T-test results for the measurement parameters of current, voltage, and power density resulted in significant differences. The significant difference in this parameter means that there is an effect on the use of ambon banana peel waste and tempeh waste as substrates of MFC

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