The effect of mango mistletoes (Dendrophthoe pentandra) leaves extract toward lmmunosenescence on old balb/c mice Available to Purchase

Immunosenescence triggers increased morbidity in geriatrics; therefore, therapeutic intervention is required. This study aims to find out the effect of mango mistletoes/Dendrophthoe pentandra (MM/Dp) towards CD4/CD8 ratio, CD4⁺CD28^- percentage, CD8⁺CD28⁻ percentage, and IL-2 level on old female mice. The mango mistletoes leaves were extracted using ethanol 96%, and then administered orally once a day for 30 days (150, 300, and 600 mg/kgBW). CD4/CD8 ratio, and percentage of CD4⁺CD28^- and CD8⁺CD28⁻ were examined using flowcytometry on the spleen sample. ELISA was performed on the blood sample extracted from the heart to study the IL-2 level. MM/Dp could lower the percentage of CD4⁺CD28^- significantly (p=0.001) (300 and 600 mg/kgBW), but statistically insignificant (p=0.121) in reducing the percentage of CD8⁺CD28^- (but has positive trend). Although MM/Dp was insignificant in increasing the IL-2 level (p=0.124), there was an increase in trend at a dose of 600 mg/kgBW (2.53 pg/ml) compared to the control group (2.02 pg/ml). The administration of various doses had a strong correlation (r=0.496) with the decreasing CD4/CD8 ratio. MM/Dp has potentials in inhibiting immunosenescence that is signified by the decreasing percentage of CD4⁺CD28⁻ and CD8⁺CD28^-, as well as increasing the IL-2 level.