Charaterization of nanocellulose from liquid wasted of tofu by Acetobacter xylinum Available to Purchase

The Research has been carried out on the use of tofu liquid waste as raw material for the manufacture of nanocellulose produced with the help of Acetobacter Xylinum bacteria. The research media was carried out by adjusting the degree of acidity to pH 4 and mixing tofu liquid waste and coconut water with a volume ratio of 1: 0; 1:1; and 3:1. The results of the study showed that at a ratio of 1: 0 no nanocellulose was formed, this was due to the absence of sufficient nutrient content in the media. Meanwhile, at a ratio of 1:1 and 3:1, nanocellulose was formed with a mass of 1.8 g and 0.9 g, respectively. The formed nanocellulose was washed with distilled water and soaked for 24 hours with 2.5% NaOH. The results were then washed again with distilled water to a neutral pH and dried using a dryer oven at a temperature of 30°C. The drying results were further characterized by FTIR, XRD, and SEM. The results of the characterization of the two samples showed the formation of nanocellulose with sizes ranging from 20 nm to 60 nm. The FT-IR results show specific groups of cellulose at wave numbers 3200-3700 cm⁻¹ for the OH group and 2900-2950 cm⁻¹ for the CH group and 998-1050 cm⁻¹ -C-O-C- group. nanocellulose is formed which is shown from the typical peaks of cellulose there are 14° and 22°.