Microbial lipid produced using yeast fermentation with in-expensive carbon sources such as hydrolysate from lignocellulosic may be an opportunity feedstock for biodiesel manufacturing. Furfural and 5-hydroxymethylfurfural (5-HMF) that can be engendered during acid hydrolysis of lignocellulose had been brought merely or concurrently into a culture medium comprising a mixture of two sugars (glucose and xylose) as carbon sources to investigate the sole inhibitory actions and their synergic enforcement on the growth and lipid production of Lipomyces starkeyi InaCC Y604. The used strain exhibited a particular resistance level against each of the archetypal inhibitor compounds. When fermented using medium-contained furfural as a sole inhibitor compound, L. starkeyi InaCC Y604 accumulated the highest microbial lipid (67.06%). While, when a mixture of furfural and 5-HMF was used as the inhibitor compounds in the fermentation medium, the microbial lipid production was achieved at 66.9%. Meanwhile, microbial lipid production was achieved at 63.68% when 5-HMF was used as the sole inhibitor in the fermentation medium. L. starkeyi InaCC Y604 showed the dexterity to tolerate inhibitors (furfural and 5-HMF) and the combination of fatty acid methyl esters (FAMEs) was unchanged by the existence of inhibitor compounds. The considerable FAMEs comply with oleic, palmitic, stearic, palmitoleic, and linoleic acids.

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