Gedi leaves (Abelmoschus manihot L.) contain many benefits, one of which is antidiabetic agents such as phenolic compound, flavonoid, and tannin. This research was conducted by adding Gedi leaves extract into Arabica coffee. The selection of coffee was based on the current trend, whereas societies commonly consume this product. The purpose of this research was to find out the best type of solvent used in extracting Gedi leaves to produce the best product (selected samples) from the formulation and compare between selected samples and synthetic medicines in their ability to lower blood glucose levels. This research consists of two-phase extraction of Gedi leaves with multiple maceration methods and in vitro test of inhibition of α-amylase (IC50), total phenolics and flavonoids test, and antioxidant activity test (IC50). Secondly, or the last, in vivo test of blood glucose levels using mice. The results of the α-amylase inhibition test (IC50), total phenolics, flavonoids, and antioxidant activity (IC50) showed that the initial 70% ethanol extract with a concentration of 50 ppm was the best sample (selected samples). When compared with synthetic medicines, the selected samples showed significantly different results, where that product had a better ability to lower blood glucose levels.

Topics
Free radicals, Flavonoid, Carbohydrates, Mammals
1.
World Health Organization (WHO)
, “
World Diabetes Day 2012 Focuses on Children's Protection
,” (
2012
).
2.
Kementerian Kesehatan RI
, “
Hari Diabetes Sedunia Tahun 2018
,” (
2019
).
3.
D. J.
Chaney
, “
Addressing the Impact of Synthetic Drugs : A Prevention Approach
,” (
2019
).
Google Scholar
 
4.
M.
Nizam
, “
Pengembalian kearifan lokal Toga sebagai jawaban atas ketergantungan obat kimia di Desa Watuagung Kecamatan Watulimo Kabupaten Trenggalek
,”
UIN Sunan Ampel Surabaya
,
2017
).
Google Scholar
 
5.
B.
Nisar
,
A.
Sultan
, and
S. L.
Rubab
, “
Comparison of medicinally important natural products versus synthetic drugs-a short commentary
,”
Nat. Prod. Chem. Res
,
6
,
308
, (
2018
).
https://doi.org/10.4172/2329-6836.1000308
Google Scholar
Crossref
Search ADS
 
6.
M.
Mutmainna
,
Y.
Purnamasari
, and
P.
Parawansah
, “
Acute Toxicity Test of Gedi (Abelmoschus manihot L.) Ethanol Leaves Extract to Histopatology of Liver Mice
,”
MEDULA
,
6
,
591
–
596
, (
2019
).
https://doi.org/10.46496/medula.v6i3.9651
Google Scholar
Crossref
Search ADS
 
7.
P.
Parawansah
, “
Activity Antidiabetic Of The Ethanol Extract Green Gedi Leaves Medical) On Mice (Mus musculus
),”
MEDULA
,
6
,
667
–
673
, (
2019
).
https://doi.org/10.46496/medula.v6i3.9653
Google Scholar
Crossref
Search ADS
 
8.
A.
Marcedes
, “
Aktivitas antidiabetes kombinasi ekstrak daun gedi merah dan daun semak bunga putih tikus induksi streptozotocin
,”
Farmakol. J. Farm.
,
14
,
159
–
166
, (
2017
).
Google Scholar
 
9.
R.
Pratiwi
,
J.
Posangi
, and
Fatimawali
, “
Uji efek analgesik ekstrak etanol daun Gedi (Abelmoschus manihot (L.) Medik) pada mencit (Mus musculus
),”
eBiomedik
,
1
,
571
–
580
, (
2013
).
Google Scholar
 
10.
M.
Masitha
, “Skrining Aktivitas Penghambatan Enzim α-Glukosidase dan Penapisan Fitoimia dari Beberapa Tanaman Obat yang Digunakan sebagai Antioksidan di Indonesia,”
Skripsi, FMIPA UI
, (
2011
).
11.
R. T.
Anggraini
 and
F. H.
Santhoso
, “
Hubungan antara Gaya Hidup Hedonis dengan Perilaku Konsumtif pada Remaja
,”
Gadjah Mada J. Psychol.
,
3
,
131
–
140
, (
2017
).
Google Scholar
 
12.
E. W.
Djuwitaningsih
, “
Perubahan Perilaku Konsumtif dan Gaya Hidup Tenaga Kerja Wanita (TKW) Purna
,”
J. Sos. Polit. Hum.
,
7
,
1
–
21
, (
2019
).
Google Scholar
 
13.
T.
Fitrilia
, “
Inhibisi Enzim Α-Glukosidase Menggunakan Ekstrak Daun Benalu Cengkeh (Dendrophthoe pentandra (L.) Mic
),”
J. Agroindustri Halal
,
3
,
41
–
47
, (
2017
).
https://doi.org/10.30997/jah.v3i1.693
Google Scholar
Crossref
Search ADS
 
14.
Y.
Haryanto
 and
V.
Kristanto
, “Optimasi Ekstraksi Senyawa Fenolik Kulit Jeruk Purut dengan Metode Ultrasound-Assisted Extraction,”
Universitas Katolik Widya Mandala Surabaya
,
2018
).
15.
S.
Subandono
, “Isolasi dan Identifikasi Flavonoid dari Daun Ceremai (Phyllanthus acidus [L.] Skeels.),”
Universitas Muhammadiyah Surakarta
,
2006
).
16.
J. A. T.
Suoth
,
S.
Sudewi
, and
D. S.
Wewengkang
, “
Analisis Korelasi Antara Flavonoid Total Dengan Aktivitas Antibakteri Ekstrak Dan Fraksi Daun Gedi Hijau (Abelmoschus manihot L
.),”
Pharmacon
,
8
,
591
–
600
, (
2019
).
https://doi.org/10.35799/pha.8.2019.29336
Google Scholar
Crossref
Search ADS
 
17.
Z.
Setiyaningrum
, “Aktivitas antiradikal dpph dan kadar fenolik dari ekstrak gambir (Uncaria gambir roxb) menggunakan metode maserasi dan soxhlet.”
Universitas Muhammadiyah Surakarta
,
2013
).
18.
I.
Indra
,
N.
Nurmalasari
, and
M.
Kusmiati
, “
Fenolik Total, Kandungan Flavonoid, dan Aktivitas Antioksidan Ekstrak Etanol Daun Mareme (Glochidion arborescense Blume
.),”
J. Sains Farm. Klin.
,
6
,
206
–
212
, (
2019
).
https://doi.org/10.25077/jsfk.6.3.206-212.2019
Google Scholar
Crossref
Search ADS
 
19.
M.
Teroreh
,
S.
Rahardjo
,
P.
Hastuti
, and
A.
Murdiati
, “
Ekstraksi daun gedi (Abelmoschus Manihot L) secara sekuensial dan aktivitas antioksidannya
,”
Agritech J. Fak. Teknol. Pertan. UGM
,
35
,
280
–
287
, (
2015
).
Google Scholar
 
20.
C. E.
Dhurhania
 and
A.
Novianto
, “
Uji Kandungan Fenolik Total dan Pengaruhnya terhadap Aktivitas Antioksidan dari Berbagai Bentuk Sediaan Sarang Semut (Myrmecodia pendens
),”
J. Farm. Dan Ilmu Kefarmasian Indones.
,
5
,
62
–
68
, (
2018
).
https://doi.org/10.20473/jfiki.v5i22018.62-68
Google Scholar
Crossref
Search ADS
 
21.
L.
Puspitasari
,
L.
Rijai
, and
H.
Herman
, “
Identifikasi Golongan Metabolit Sekunder dan Aktivitas Antioksidan Eksstrak Daun Brotowali (Tinospora tuberculata Beumee
),”
Saintech Farma
,
11
,
18
–
24
, (
2018
).
Google Scholar
 
22.
J. K. S.
Lung
 and
D. P.
Destiani
, “
Uji aktivitas antioksidan vitamin A, C, E dengan metode DPPH
,”
Farmaka
,
16
,
53
–
62
, (
2017
).
Google Scholar
 
23.
Irnawati
,
Mirnawati
Purba
,
R.
Mujadilah
, and Sarmayani, “
Penetapan kadar vitamin C DAN UJI aktifitas antioksidan sari buah songi (Dillenia serrata Thunb.) terhadap radikal DPPH (Diphenylpicrylhydrazyl
),”
Pharmacon
,
6
,
40
–
44
, (
2017
).
Google Scholar
 
24.
F.
Assagaf
,
A.
Wullur
, and
A.
Yudistira
, “
Uji Toksisitas Akut (Lethal Dose50) Ekstrak Etanol Daun Gedi Merah (Abelmoschus manihot L.) Terhadap Tikus Putih Jantan Galur Wistar (Rattus norvegicus L
.),”
Pharmacon
,
2
,
23
–
28
, (
2013
).
Google Scholar
 
25.
A.
Anjeli
, “Studi Literatur Senyawa Antioksidan Dalam Tanaman Obat Terhadap Penurunan Kadar Gula Darah.”
Universitas_Muhammadiyah_Mataram
,
2020
).
26.
J.
Tandi
,
H. Z.
Muthi'ah
,
Y.
Yuliet
, and
Y.
Yusriadi
, “
Efektivitas Ekstrak Daun Gedi Merah terhadap Glukosa Darah, Malondialdehid, 8-Hdroksi-Deoksiguanosin, Insulin Tikus Diabetes
,”
J. Trop. Pharm. Chem.
,
3
,
264
–
276
, (
2016
).
https://doi.org/10.25026/jtpc.v3i4.114
Google Scholar
Crossref
Search ADS
 
27.
I.
Irdalisa
,
S.
Safrida
,
K.
Khairil
,
A.
Abdullah
, and
M.
Sabri
, “
Profil kadar glukosa darah pada tikus setelah penyuntikan aloksan sebagai hewan model hiperglikemik
,”
J. EduBio Trop.
,
3
,
1
–
50
, (
2015
).
Google Scholar
 
28.
E.
Esmawati
, “Pengaruh ekstrak daun sirsak (Annona murica L.) terhadap kadar glukosa darah dan hitologi pankreas tikus (Rattus norvegicus) yang diinduksi aloksan.”
Universitas Islam Negeri Maulana Malik Ibrahim
,
2015
).
29.
A.
Yuriska
 F, “Efek Aloksan Terhadap Kadar Glukosa Darah Tikus Wistar,” (
Universitas Diponegoro
,
Universitas Diponegoro
,
2009
).
30.
P.
Satriany
, “
Pengaruh pemberian ekstrak herba daun sendok (Plantago Major L.) terhadap kadar glukosa darah mencit Balb/C induksi Streptozotocin
,” (
2010
).
Google Scholar
 
31.
R.
Widiastuti
 and Kristianti, “Uji Penurunan Kadar Glukosa Darah Ekstrak Etanol Herba Daun Sendok (Plantago mayor L.) Pada Kelinci Jantan Yang Dibebani Glukosa.”
Universitas Muhammadiyah Surakarta
,
2008
).
32.
A.
Rahmawati
, “
Pengaruh Profil Responden Terhadap Pemilihan Obat Herbal Dan Obat Kimia Sintetis Di Kelurahan Wuryorejo Kabupaten Wonogiri
,” (
2014
).
Google Scholar
 
33.
W. M.
Sidoretno
 and
I. O.
Rz
, “
Edukasi Bahaya Bahan Kimia Obat yang Terdapat Didalam Obat Tradisional
,”
J. Pengabdi. Masy. Multidisiplin
,
1
,
123
–
177
, (
2018
).
Google Scholar
 
This content is only available via PDF.
©2023 Authors. Published by AIP Publishing.
2023
Author(s)
You do not currently have access to this content.