Insertion sequence 256 element detect in MRSA bacterial isolates, transposition in genomic of bacteria elicited change in resistance of S. aureus. IS256 is affect a bout to the resist of antibiotics, this influence detects by exposure S. aureus (that it has contain IS256 in their genome) under stress ciprofloxacin antibiotic. Staphylococcus aureus, Clinical MRSA isolate from nosocomial infection was sampling in wound, identification by compound system Vitek -2 and has resistance to 11(78.5%) antibiotics used in this study, then measured MIC and MBC to Ciprofloxacin 16 µg/ml, using obligate primer and amplified of IS256 in bacterial isolate by using PCR system. S. aureus with a natural insertion of IS256 was cultured with Ciprofloxacin at sub-inhibitory doses applied on brain heart infusion agar and pouring each six hours then a counting transposition frequency and percentage survival fraction put in analysis statistical significance, and impact of study was Ciprofloxacin increased the transposition frequency in bacterial isolate and decreased percentage survival fraction to all antibiotics used in this study and S. aureus had resistance of them.

Topics
Diseases and conditions, Genomics, Bacteria, Antibiotics
1.
F. S.
Szekat
,
C.
Josten
,
M.
Georg
, H. S., and
Bierbaum
,
G.
 “,
Antibiotic-Induced Autoactivation of IS256 in Staphylococcus aureus
,” in.
Antimicrobial Agents and Chemotherapy.
Vol.
57
, no.
12
, pp.
6381
–
6384
.
2013
.
https://doi.org/10.1128/AAC.01585-13
Google Scholar
Crossref
Search ADS
PubMed
 
2.
D. Thapaliya.
“
,
Prevalence and Molecular Characterization of S. aureus and MARSA on Children’s Playgrounds
,”.
The Pediatric Infectious Disease Journal
Vol.
38
, no.
3
May
2018
.
Google Scholar
 
3.
S.Y.
Tong
,
J.S
Davis
,
E.
Eichenberger
,
T.L.
Holland
,
Fowler.
“
,
Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifections, and management
,”.
Clin Microbiol Rev.
Vol.
28
, no.
3
, pp:
603
–
661
.
2015
.
https://doi.org/10.1128/CMR.00134-14
Google Scholar
Crossref
Search ADS
PubMed
 
4.
F.
Schaumburg
,
G.
Peters
,
A.
Alabi
 et al “,
Afr. Missense mutations of PBP2a are associated with reduced susceptibility to ceftaroline and ceftobiprole in ican MRSA
,”.
J Antimicrobe Chemoth.
Vol:
71
; pp.
41
–
4
.
2016
.
https://doi.org/10.1093/jac/dkv325
Google Scholar
Crossref
Search ADS
 
5.
D.
Gregorio
,
S.
Fernandez
,
S.
Perazzi
,
B.
Bello
,
N.
Famiglietti
,
A. Mollerach.
“
,
Increase in IS256 transposition in invasive vancomycin heteroresistant Staphylococcus aureus isolate belonging to ST100 and its derived VISA mutants,” NCBI
.
US National Library of Medicine National Institutes of Health
.
2016
.
Google Scholar
 
6.
J.H.
Moffatt
,
M.
Harper
,
B.
Adler
,
R.L.
Nation
,
J.
Li
,
J.D. Boyce
“
,
Insertion sequence ISAba11 is involved in colistin resistance and loss of lipopolysaccharide in Acinetobacter baumannii,
”
antimicrobe Agents Chemother.
Vol.
55
, pp.
3022
–
3024
2011
.
https://doi.org/10.1128/AAC.01732-10
Google Scholar
Crossref
Search ADS
 
7.
J.
Vandecraen
,
M.
Chandler
,
A.
Aertsen
, and
V. Houdt
“
,
The Impact of Insertion Sequences on Bacterial Genome Plasticity and Adaptability
,”
Critical Reviews in Microbiology.
Vol.
43
, no.
6
, pp.
709
–
730
.
2017
.
https://doi.org/10.1080/1040841X.2017.1303661
Google Scholar
Crossref
Search ADS
PubMed
 
8.
Clinical and Laboratory Standards Institute (CLSI)
.
Performance standards for antimicrobial susceptibility testing: Twenty-second Informational Supplement
.
CLSI document
, Vol.
100
, no.
22
.
2016
.
9.
C. I. Mowuama
“
,
Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a novel dilution tube method
,”
African Journal of Microbiology Research
. Vol.
11
, no.
23
, pp.
977
–
980
.
2017
.
Google Scholar
 
10.
S. K.
Kyoung
,
S. W.
Yuna
,
J.
Juho
,
K.
Hur
,
K.H.
Park
,
J. S.
Kim
,
D. Kim.
“
,
Memrane-targeting Triphenylphs-phonium Functionalized Ciprofloxacin for Methicillin-Resistant Staphylococcus aureus (MRSA)
,”
MDPI, Antibiotics.
Vol.
9
, no.
11
, pp.
758
.
2020
.
https://doi.org/10.3390/antibiotics9110758
Google Scholar
Crossref
Search ADS
 
11.
Gleeson
,
P.
Larkin
,
C.
Walsh
,
N.
Ó
Sullivan
 “,
Methicillin-Resistant Staphylococcus aureus: Prevalence, incidence, risk factors, and effects on survival of Patients in a Specialist Palliative Care Unit: A prospective Observational study
,”
SAGA journals. Palliative Medicine.
2015
.
Google Scholar
 
12.
M.
Nagel
,
T.
Reuter
,
A.
Jansen
,
C.
Szekat
,
B. Gabriele
“
,
Influence of Ciprofloxacin and vancomycin on mutation rate and transposition of IS 256 in Staphylococcus aureus,
”
ELSEVIER, International Journal of Medical Microbiology.
Vol.
301
, IS.
3
, pp.
229
–
236.2.11
.
https://doi.org/10.1016/j.ijmm.2010.08.021
Crossref
Search ADS
 
13.
S. Franziska
“
,
An Elevated Mutation Frequency Favors Development of Vancomycin Resistance in Staphylococcus
14.
aureus,”
Antimicrobial Agents and Chemotherapy
. vol.
46
, IS.
11
, pp.
3540
.
2002
.
https://doi.org/10.1128/AAC.46.11.3540-3548.2002
Crossref
Search ADS
PubMed
 
15.
SAS. Statistical Analysis System, User’s Guide
. Statistical. Version 9.1th ed.
SAS. Inst. Inc. Cary. N.C
.
USA
.
2012
.
PubMed
 
16.
S.
Sanguinetti
,
M.
Ciccaglione
,
D.
D´Inzeo
,
D.
Romano
,
L.
Leone
,
F. Fadda
“
,
Use of the Vitek 2 System for Rapid Identification of Clinical Isolates of Staphylococci from Bloodstream Infections
,”
American Society for Microbiology, Journal of Clinical Microbiology.
Vol.
41
, no.
9
, pp.
4259
–
4263
.
2003
.
https://doi.org/10.1128/JCM.41.9.4259-4263.2003
Google Scholar
Crossref
Search ADS
 
17.
W. K.
Ziebuhr
,
D. M.
Trautamann
,
M. Wilhelm
“
,
Chromosomal rearrangements affecting biofilm production and antibiotic resistance in a Staphylococcus epidermidis strain causing shunt-associated ventriculitis
,”
Int.J. Med. Microbial.
Vol.
290
, pp.
115
–
120
.
2000
.
https://doi.org/10.1016/S1438-4221(00)80115-0
Google Scholar
Crossref
Search ADS
 
18.
J.H.
Moffatt
,
M.
Harper
,
B.
Adler
,
R.L.
Nation
,
J.
Li
,
J.D. Boyce
“
,
Insertion sequence ISAba11 is involved in colistin resistance and loss of lipopolysaccharide in Acinetobacter baumannii,
”
Antimicrob Agents Chemother.
Vol.
55
, pp.
3022
–
3024
.011
https://doi.org/10.1128/AAC.01732-10
Crossref
Search ADS
PubMed
 
19.
C. I.
Mowuama“
,
Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a novel dilution tube method
,”
African Journal of Microbiology Research.
Vol.
1
1, no.
23
, pp.
977
–
980
.
2017
.
Google Scholar
 
20.
C.
Kozitskaya
,
H.S.
Cho
,
K. D.
Reinhared
,
M.
Kurt
,
W. N. Ziebuhr
“
,
The bacterial Insertion Sequence Element IS256 Occurs Preferentially in Nosocomial Staphylococcus epidermidis Isolater: Association with Biofilm Formation and Resistance to Aminoglycosides
,”
American Society for Microbiology.
Vol.
72
, no.
2
, pp.
1210
–
1215
.
2004
.
Google Scholar
 
21.
G. A.
Larkin
,
P.
Walsh
,
C. N. ÓSullivan
“
,
Methicillin-Resistant Staphylococcus aureus: Prevalence, incidence, risk factors, and effects on survival of Patients in a Specialist Palliative Care Unit: A prospective Observational study
,”
SAGA journals. Palliative Medicine.
2015
.
Google Scholar
 
22.
M.
Mohammed
,
N.
Bahrami
,
M.
Khajavian
,
J. Faghri
“
,
The Occurrence of Type I, II and III Integrons in multidrug resistance and Methicillin-resistance Staphylococcus aureus isolates in Iran
,”
Springer link.
Vol.
77
, no:
147
, pp.
1653
–
1659
.
2020
.
Google Scholar
 
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