Identification of bioactive compounds from black pepper and turmeric extracts and their antioxidant, antibacterial activity structural confirmation by GC-MS and FT-IR for feed formulation Available to Purchase

In this research the study evaluates the identification of bioactive compounds, antioxidant and antimicrobial activities of black pepper and turmeric extract for structural confirmation by GC-MS and FT-IR. Black pepper and turmeric were purchased from the local market and dried then pulverized into fine powder. The antioxidant activities were assessed by standard protocol and zone of inhibition was observed for well and disc diffusion against E.coli as per the procedure. Identification of bioactive compounds are confirmed by GC-MS and FT-R. Independent sample T test was done which revealed that the nanoformulated blackpepper and turmeric extract appeared to have higher amount of flavonoids (158.5±10.5) mgGAE/g, polyphenols (93.67±6.07) mgGAE/g. (P<0.005). The nanoformulated extract showed comparatively good results against E.coli with respect to control antibiotics. The nanoformulated extract showed the effective antibacterial and found to be the highest zone of inhibition of 12±1mm for ethanol based extract and 14±1mm for antibiotic respectively. Antioxidant activities and antibacterial activity of nanoformulated blackpepper and turmeric extract was determined and presence of bioactive compounds was analysed and confirmed by GC-MS and FT-IR.