Determination of bactericidal effect of piper betel leaf extract on bacteria by filter paper disc method to check antimicrobial efficacy

Effect of a natural compound extracted from Piper Betel leaf by determining bactericidal and or bacteriostatic effect to check antimicrobial efficacy on the pathogenic microorganisms. Bioactive compound extracted in different solvents i.e distilled water, ethanol, and methanol, and its effectivity was determined by measuring the zone of clearance (1 group and 96 samples per group by keeping threshold 0.05 and G power 80%, coincidence interval 95%, and enrollment ratio as 1) by agar diffusion method using kanamycin (30) as a control for the different time period (24 Hrs, 48Hrs and 72Hrs) at 37°C. Promising results were found for the zone of clearance in Escherichia.coli with a significance of about P<0.001 in the ethanol extract after 72 hours of incubation. Compared to 10% extract application where growth of S.aureus was reduced to 4%, addition of 100% crude inhibits bacterial growth up to 95% approximately. For S.aureus and E.coli though ethanol extract was found effective however no significant effect was observed for inhibiting Pseudomonas. Novel bioactive compound extracted from Piper Betel leaf was found effective against pathogens and can be used as a natural component with antimicrobial efficacy in the field of medicine.