Legin is an inoculum containing bacteria that can fix N2 in the air. The activity of Bradyrhizobium japonicum in legin was influenced by the availability of macro and micronutrients supplied from cow urine liquid fertilizer. This study aimed to determine the effect of legin inoculation and cow urine liquid fertilizer on N uptake and yield of soybean (Glycine max (L.) Merill. var. dega 1). The research was carried out from January to April 2021 in.Ngabean Village, Secang District, Magelang Regency with an altitude of 470 m above sea level with latosol soil type. The research design was a Completely Randomized Block Design (RAKL) with 2 treatment factors and 4 replications. The first factor was legin inoculation with doses of 0, 10, 20, 30, 40 g/kg. The second factor is the treatment of cow urine liquid fertilizer 0 ml/l water and treatment of cow urine liquid fertilizer 100 ml/l water. Data were analyzed with variance and continued with Orthogonal Polynomial Test and 1 % LSD Test. The results showed that the legin inoculation treatment increased the number of root nodules, N uptake and number of filled pods. Treatment of cow urine increased the number of root nodules, N uptake and number of filled pods. The interaction between legin inoculation and cow urine treatment did not affect all parameters of the observation. Inoculation of legin 20 g/kg and cow urine 100 ml/L gave the highest results for all parameters observed.

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