Biduri Calotropics gigantea belongs to the Asclepiadaceae family and belongs to the shrub plant. Biduri seeds and stems produce fibers that can be used as raw materials for non-clothing textiles, technical textiles, and functional textiles. The quality and quantity of fiber produced by biduri is determined by the cultivation technique used, one of the most influential cultivation techniques is fertilization. This study discusses the effect of the application of vermicompost and mycorrhizal fungi on the growth and yield of biduri. This study used the randomized complete block design (RCBD) experimental design with analysis of variance (ANOVA) and DMRT (ducan multiple range test) at the 5% level. The first factor is the dose of vermicompost fertilizer (K) which has 4 levels: K0 = 0 ton/ha, K1 = 10 tons/ha, K2 = 20 tons/ha, and K3 = 30 tons/ha and the second factor is dose of arbuscular mycorrhizal fungi (C) in 4 level C0 = 0 g, C1 = 5 g, C2 = 10 g, and C3 = 15 g per plant. The result of this research are dose of vermicompost fertilizer gives a significant effect on the variables plant height, number of leaves, stem diameter, leaf area, number of stomata, stomata width, and leaf greenness. Dose of arbuscular mycorrhizal fungi (AMF) did not give significant effect to all growth variables of C. gigantea. The combination of dose of vermicompost fertilizer and dose of arbuscular mycorrhizal fungi did not give a significant effect on the all variables.

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