Cervical cancer is the second leading cause of cancer death in women in developing countries. Various ways of treating cervical cancer continue to be developed, one of which is the discovery of cytotoxic agents. Sargassum sp is one of macroalgae which presumably has a potential as a chemopreventive agents because of its secondary metabolites antiprolifcontains antioxidant, antihyperglycemic, and anticancer activities. This study aims to determine the effect of taurine and ethanol extraction from Sargassum sp. on cytotoxic activity and antiproliferation effect on HeLa cell lines. In this study, phytochemical testing was carried out to determine the content of secondary metabolites in the ethanol extraction of Sargassum. The concentration of taurine and ethanol extract of Sargassum sp. used was 625 ppm, 1250 ppm, 2500 ppm and 5000 ppm, respectively. Evaluation of cell viability using IC50 values was determined by 3-(4,5-Dimethylthiazole-2-yl)-2,5-Diphenyl Tetrazolium Bromide (MTT Assay) method. The anti-proliferation test was determined by the doubling time value. Data were analyzed by One Way ANOVA using SPSS at the 95% confidence level, and followed by the Least Significant Difference (LSD) test. The results showed that taurine did not have an IC50 value. The ethanol extract of Sargassum sp., respectively, showed a cytotoxic effect with IC50 values of 1915 ppm. The doubling time value of taurine, respectively, from a concentration of 625-5000 ppm were 161, 154, 148, 124 hours longer than those in control cell, while the ethanol extracts of Sargassum sp. was 106 respectively at a concentration of 625 ppm, whereas at concentrations above 1250 ppm, there were no doubling time value, presumably that the HeLa cells have experienced death. Based on the results of this study, it can be concluded that taurine and ethanol extracts of Sargassum sp. have the potential to be developed as anticancer agents in HeLa cervical cancer treatment.

Topics
Organic compounds, Free radicals, Diseases and conditions, Cell viability, Flavonoid, Cancer treatment, Cell lines, Analysis of variance
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