This study at to determine the content of phytochemical compounds in Indigofera longeracemosa. The analysis was carried out on a sample of I. longeracemosa from Kulon Progo, Central Java. In previous studies, metabolites of the genus Indigofera were found to exhibit various bioactivities including antimicrobial, insecticidal, phytotoxic, hepatotoxic, teratogenic and cytotoxic. Qualitative and quantitative phytocomponent tests were carried out in I. longeracemosa. The content of several important phytochemicals such as phenols, tannins, flavonoids, terpenoids, carbohydrates and proteins of I. longeracemosa was studied using spectrophotometric methods. Extracts from fresh dried leaves of I. longeracemosa were tested for phytochemical screening and TLC for determination of phytoconstituents. The results showed that laboratory tests of the content of I.longeracemosa leaves from 3 regions of Magelang, Trisik, and Srandakan contained flavonoids and saponins. This data can be used as a reference for further research with specimens from a wider area or references for other researchers.

Topics
Java, Antimicrobials, Proteins, Flavonoid, Carbohydrates, Bioactivity
1.
B2P2TOOT
.
Screening and Assay Work Instructions
. (
B2P2TOOT
,
Tawangmangu
,
2019
).
2.
Culvenor
,
CCJ
, &
Fitzgerald
,
JS
.
A Field Method for Alkaloid Screening of Plants
.
Journal of Pharmaceutical Sciences
, vol.
52
, iss.
3
, p.
303
–
304
(
1963
).
https://doi.org/10.1002/jps.2600520327
Google Scholar
 
3.
Esimon
,
C.O.A
,
Dikwu
,
M.U
 and
Muko
,
K.N.
 
Antibacterial properties of Indigofera leaves
.
Fitroterapia
, vol.
70
, p.
517
–
520
(
1999
).
https://doi.org/10.1016/S0367-326X(99)00072-6
Google Scholar
 
4.
Farnsworth
,
NR
.
Biological and Phytochemical Screening of Plants
.
Journal of Phramaceucatical Science
, vol.
55
, iss.
3
, p.
245
–
259
(
1996
).
Google Scholar
 
5.
Harborne
,
JB
.
Phytochemical Method. Translated by Sujatmi
. (
ITB Press
,
Bandung
,
1987
), p.
9
–
13
.
Google Scholar
 
6.
Haryanto
,
D.
, Bagumono and Suyadi.
Cultivation of Indigofera as Raw Material for Natural Batik Dye
.
Regional Research Journal
, vol.
14
, iss.
3
, (
2015
).
Google Scholar
 
7.
Hidayah
,
WW
,
Kusrini
,
D.
, &
Fachriyah
,
E.
 
Isolation
,
Identification of Steroid Compounds from Getih-Getihan Leaves (Rivina humilis L.) and Activity Test as Antibacterial
.
Journal of Science And Application Chemistry
, vol.
19
, iss.
1
, p.
32
–
37
(
2016
).
Google Scholar
 
8.
Itzhak
 
B.
,
Wexler
 
H.M
,
Goldstein
 
J.C.E.
 
Antianaerobic Antimicrobials: Spectrum and Susceptibility Testing
.
Clin Microbiol Rev. July
, vol.
26
, iss.
3
, p.
526
–
546
(
2013
).
https://doi.org/10.1128/CMR.00086-12
Google Scholar
 
9.
Kristanti
 et al. 
Phytochemistry textbook
. (
Erlangga Publisher
,
Surabaya
,
2018
).
Google Scholar
 
10.
Marliana
,
SD
,
Suryanti
,
V.
, &
Suyono
.
Phytochemical Screening and Thin Layer Chromatographic Analysis of Chemical Components of Chayote (Sechium edule Jacq. Swartz.) in Ethanol Extract
.
Biopharmaceuticals
, vol.
3
, iss.
1
, p.
26
–
31
(
2005
).
Google Scholar
 
11.
Mishra
,
Debendranath
 &
Gomare
,
Komal
.
GC-MS Analysis and Phytochemical Screening of Indigofera tinctoria (Linn.) Leaf Extract Characterizing its Medicinal Use
.
International Journal of Ayurvedic Medicine
, vol.
11
, iss.
2
, p.
289
–
299
(
2020
).
https://doi.org/10.47552/ijam.v11i2.1540
Google Scholar
 
12.
Oluwafemi
,
R.A.
,
Isiaka
 
Olawale
 and
Alagbe
,
J.O.
 
Recent trends in the utilization of medicinal plants as growth promoters in poultry nutrition-A review
.
Agricultural and Veterinary Sciences
, vol.
4
, iss.
1
, p.
5
(
2020
).
Google Scholar
 
13.
Prakash
,
M.
,
Suri
,
S.
,
Upadhyay
,
G
 and
Singh
,
S.
 
Total Phenolic Free Radical Scavenging Activities of Some Medicinal Plants
.
Int.J. Food. Sci. Nutr.
, vol.
58
, p.
1
–
9
(
2007
).
https://doi.org/10.1080/09637480601093269
Google Scholar
 
14.
Saito
,
S.
 
Knowledge of Engineering Materials
. (
PT Pradnya Paramita
,
Jakarta
,
2005
).
Google Scholar
 
15.
Tamilselvi
,
N.
,
Krishnamoorthy
,
P.
,
Dhamotharan
,
R.
,
Arumugam
,
P.
, and
Sagadeva
,
E.
 
Analysis of total phenols, total tannins and screening of phytocomponennts in Indigofera asphaltathoides (Shivanar Vembu) Vahl EX DC
.
Journal of Chemical and Pharmaceutical Research
, vol.
4
, iss.
6
, p.
32
(
2012
).
Google Scholar
 
16.
Vinayak
 
RC
,
Sabu
 
AS
,
Chatterji
 
A.
,
Bioprospecting of a few brown seaweeds for their cytotoxic and antioxidant activity
.
Complementary and Alternative Medicine
, vol.
2011
, p.
1
–
9
(
2010
).
Google Scholar
 
17.
Yusuf
,
AM
.
Quantitative, Qualitative & Joint Research Methods
. (
Kencana
,
Jakarta
,
2014
).
Google Scholar
 
This content is only available via PDF.
© 2023 Author(s).
2023
Author(s)
You do not currently have access to this content.