Fuzzy recognition of proteins in 2D electrophoresis in population genetics Available to Purchase

The development of the previously created technique of fuzzy recognition of proteins in population genetics during gel electrophoresis for the case of 2D electrophoresis is proposed. For a more complete separation of proteins with a similar mass in a mixture, they are first subjected to isoelectric focusing in one direction, and then electrophoresis is carried out in the perpendicular direction. Isoelectric focusing is a type of zone electrophoresis that separates protein molecules based on the difference in their isoelectric points. Localization of proteins after isoelectric focusing is uneven; in addition, the movement of proteins during electrophoresis occurs no longer along a straight cuvette, but in a plane. All this introduces additional distortions in the numerical determination of the mass of proteins. The technique we have developed involves the construction of an experimental membership function for 2D electrophoresis, its approximation by a Gaussian or polynomial surface, and, then, determination of the fuzzy mass of proteins. The features of fuzzy protein recognition for 2D electrophoresis are considered.