Silver nanoparticles were found to have an excellent antimicrobial activity and green synthesis enhance the activity of Silver nanoparticles. Mimosa pudica commonly called as touch me-not plant has found to have antimicrobial, anti-oxidant, anti-inflammatory and antiseptic properties. Ormocarpum cochinchinense commonly called as ‘elumbotti’ is a shrub which is traditionally used for bone fixing in the areas like Tamilnadu .Hydroxyapatite is one of the popular biomaterial which has good biocompatibility commonly used in the field of dentistry and Orthopaedics. Osteosarcoma or Osteogenic sarcoma is a primary malignant bone tumour commonly affects children and adolescents. Recently, Hydroxyapatite was found to have proliferative-suppressive effect on osteosarcoma cells. The leaves of Mimosa pudica and ormocarpum cochin chinense were shadow dried and its aqueous extract was prepared. The Silver nano particles were synthesized from both the plants and the UV-visible spectrum shows good stability in mimosa pudica sample which was taken for further use. Further the sample was characterized by Transmission Electron Microscopy (TEM) and Energy dispersive X-ray analysis (EDX), X-ray Diffraction (XRD).To enhance the drug delivery, synthesized silver nanoparticles were conjugated with hydroxyapatite powder. The antimicrobial and cytotoxic activity of the bio-conjugate was evaluated. The in-vitro activity of the bio conjugate will be evaluated in the osteo sarcoma cell line (MG-63). The produced bio conjugate can be used in the treatment of osteo sarcoma and in treating bone injuries and bone inflammations.
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