Infection of conventional mice with chimeric HIV-1, EcoHIV, have been used for study of systemic HIV-1 infection, antiviral immune responses and neuroinvasiveness. Chimeric virus EcoHIV was constructed by replacement of the gp120 region in HIV-1 with the envelope gene gp80 from ecotropic murine leukemia virus (MLV) that infects only rodent cells. For EcoHIV infection of human cell lines, mouse high affinity cationic amine acid transporter 1, mCAT1, a receptor for ecotropic MLV, was stably expressed in Hela cell line. The resulting Hela-mCAT1 cell line was successfully and efficiently infected by EcoHIV. EcoHIV infection of human heterogenic cell lines greatly recapitulate the characteristics of HIV-1 infection in human beings for convenient and safe study of HIV-1IAIDS.

1.
Gottlieb
,
M.S.
, et al.,
Pneumocystis carinii pneumonia and mucosal candidiasis in previously healthy homosexual men: evidence of a new acquired cellular immunodeficiency
.
N Engl J Med
,
1981
.
305
(
24
): p.
1425
31
.
2.
Schwetz
,
T.A.
and
A.S.
Fauci
,
The Extended Impact of Human Immunodeficiency VirusIAIDS Research
.
J Infect Dis
,
2019
.
219
(
1
): p.
6
9
.
3.
Broder
,
S.
,
The development of antiretroviral therapy and its impact on the HIV-1IAIDS pandemic
.
Antiviral Res
,
2010
.
85
(
1
): p.
1
18
.
4.
Gulick
,
R.M.
and
C.
Flexner
,
Long-Acting HIV Drugs for Treatment and Prevention
.
Annu Rev Med
,
2019
.
70
: p.
137
150
.
5.
Volberding
,
P.A.
and
S.G.
Deeks
,
Antiretroviral therapy and management of HIV infection
.
Lancet
,
2010
.
376
(
9734
): p.
49
62
.
6.
Deeks
,
S.G.
,
R.
Tracy
, and
D.C.
Douek
,
Systemic effects of inflammation on health during chronic HIV infection
.
Immunity
,
2013
.
39
(
4
): p.
633
45
.
7.
Potash
,
M.J.
, et al.,
A mouse model for study of systemic HIV-1 infection, antiviral immune responses, and neuroinvasiveness
.
Proc Natl Acad Sci U S A
,
2005
.
102
(
10
): p.
3760
5
.
8.
Gu
,
C.J.
, et al.,
EcoHIV infection of mice establishes latent viral reservoirs in T cells and active viral reservoirs in macrophages that are sufficient for induction of neurocognitive impairment
.
PLoS Pathog
,
2018
.
14
(
6
): p.
e1007061
.
9.
Geraghty
,
P.
, et al.,
HIV infection model of chronic obstructive pulmonary disease in mice
.
Am J Physiol Lung Cell Mol Physiol
,
2017
.
312
(
4
): p.
L500
L509
.
10.
Yin
,
C.
, et al.,
In Vivo Excision of HIV-1 Provirus by saCas9 and Multiplex Single-Guide RNAs in Animal Models
.
Mol Ther
,
2017
.
25
(
5
): p.
1168
1186
.
11.
Hadas
,
E.
, et al.,
Testing antiretroviral drug efficacy in conventional mice infected with chimeric HIV-1
.
Aids
,
2007
.
21
(
8
): p.
905
9
.
12.
Bai
,
L.
, et al.,
CAT1ISLC7A1 acts as a cellular receptor for bovine leukemia virus infection
.
FASEB J
,
2019
.
33
(
12
): p.
14516
14527
.
13.
Albritton
,
L.M.
, et al.,
Envelope-binding domain in the cationic amino acid transporter determines the host range of ecotropic murine retroviruses
.
J Virol
,
1993
.
67
(
4
): p.
2091
6
.
14.
Yoshimoto
,
T.
,
E.
Yoshimoto
, and
D.
Meruelo
,
Identification of amino acid residues critical for infection with ecotropic murine leukemia retrovirus
.
J Virol
,
1993
.
67
(
3
): p.
1310
4
.
15.
Kakoki
,
K.
, et al.,
Susceptibility of muridae cell lines to ecotropic murine leukemia virus and the cationic amino acid transporter 1 viral receptor sequences: implications for evolution of the viral receptor
.
Virus Genes
,
2014
.
48
(
3
): p.
448
56
.
16.
Bae
,
E.H.
,
S.H.
Park
, and
Y.T.
Jung
,
Role of a third extracellular domain of an ecotropic receptor in Moloney murine leukemia virus infection
.
J Microbiol
,
2006
.
44
(
4
): p.
447
52
.
17.
Albritton
,
L.M.
, et al.,
A putative murine ecotropic retrovirus receptor gene encodes a multiple membrane-spanning protein and confers susceptibility to virus infection
.
Cell
,
1989
.
57
(
4
): p.
659
66
.
18.
Kim
,
J.W.
, et al.,
Transport of cationic amino acids by the mouse ecotropic retrovirus receptor
.
Nature
,
1991
.
352
(
6337
): p.
725
8
.
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