Infection of conventional mice with chimeric HIV-1, EcoHIV, have been used for study of systemic HIV-1 infection, antiviral immune responses and neuroinvasiveness. Chimeric virus EcoHIV was constructed by replacement of the gp120 region in HIV-1 with the envelope gene gp80 from ecotropic murine leukemia virus (MLV) that infects only rodent cells. For EcoHIV infection of human cell lines, mouse high affinity cationic amine acid transporter 1, mCAT1, a receptor for ecotropic MLV, was stably expressed in Hela cell line. The resulting Hela-mCAT1 cell line was successfully and efficiently infected by EcoHIV. EcoHIV infection of human heterogenic cell lines greatly recapitulate the characteristics of HIV-1 infection in human beings for convenient and safe study of HIV-1IAIDS.
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26 July 2022
4TH INTERNATIONAL CONFERENCE ON FRONTIERS OF BIOLOGICAL SCIENCES AND ENGINEERING (FBSE 2021)
29–30 September 2021
Jinan City, China
Research Article|
July 26 2022
Overexpression of mCAT1 on human heterogenic cell lines for EcoHIV infection
Hao Hu;
Hao Hu
a)
1
College of Life and Health Sciences, Institute of Biology and Medicine, Wuhan University of Science and Technology
, Wuhan, 430000, China
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Jiahao Chen;
Jiahao Chen
b)
1
College of Life and Health Sciences, Institute of Biology and Medicine, Wuhan University of Science and Technology
, Wuhan, 430000, China
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Mengdan Song;
Mengdan Song
c)
1
College of Life and Health Sciences, Institute of Biology and Medicine, Wuhan University of Science and Technology
, Wuhan, 430000, China
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Qiying Yu;
Qiying Yu
d)
1
College of Life and Health Sciences, Institute of Biology and Medicine, Wuhan University of Science and Technology
, Wuhan, 430000, China
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Yan Ma;
Yan Ma
e)
1
College of Life and Health Sciences, Institute of Biology and Medicine, Wuhan University of Science and Technology
, Wuhan, 430000, China
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Zhihong Song;
Zhihong Song
f)
1
College of Life and Health Sciences, Institute of Biology and Medicine, Wuhan University of Science and Technology
, Wuhan, 430000, China
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Haifei Shang;
Haifei Shang
g)
1
College of Life and Health Sciences, Institute of Biology and Medicine, Wuhan University of Science and Technology
, Wuhan, 430000, China
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Xuejiao Xia;
Xuejiao Xia
h)
1
College of Life and Health Sciences, Institute of Biology and Medicine, Wuhan University of Science and Technology
, Wuhan, 430000, China
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Chaojiang Gu
Chaojiang Gu
i)
1
College of Life and Health Sciences, Institute of Biology and Medicine, Wuhan University of Science and Technology
, Wuhan, 430000, China
i)Corresponding author email: [email protected]
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AIP Conf. Proc. 2511, 020009 (2022)
Citation
Hao Hu, Jiahao Chen, Mengdan Song, Qiying Yu, Yan Ma, Zhihong Song, Haifei Shang, Xuejiao Xia, Chaojiang Gu; Overexpression of mCAT1 on human heterogenic cell lines for EcoHIV infection. AIP Conf. Proc. 26 July 2022; 2511 (1): 020009. https://doi.org/10.1063/5.0093972
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