Synthesis of 4-(3-(4-chlorophenyl)-1-phenyl-4,5-dihydro-1H-pyrazol-5-yl)-2-methoxyphenol as antibacterial agent was performed by cycloaddition reaction. These compound was performed by refluxing the chalcone with phenylhydrazine in acidic condition for 4 h. The structure of the synthesized compound was confirmed by FTIR and 1H-NMR spectrometers. The pyrazoline was screened for antibacterial activity by agar well-diffusion against Staphylococcus aureus, Bacillus cereus, Bacillus subtillis, Escherichia coli and Shigella flexnerri. The pyrazoline was found to exhibits an antibacterial activity and its zone of inhibition/concentration against Staphylococcus aureus (4.75/100), Bacillus cereus (2.50/500), Bacillus subtillis (4.50/300), Escherichia coli (4.75/500) and Shigella flexnerri (3.75/300). The result showed that the pyrazoline which were subtituted with chloro, hydoxy, and methoxy, was able to inhibit the growth of the tested pathogenic bacteria.

1.
Rinasih
,
I.
1998
Sintesis Senyawa 3,4-dimetoksibenzaldehid dari Vanilin dalam Suasana Basa Skripsi
FMIPA UGM
Yogyakarta
.
2.
Lina
Fauzi’ah
and
Tutik Dwi
W.
2017
Synthesis of 1-phenyl-3-(4’-nitrophenyl)-5-(3’,4’-dimethoxy-6’-nitrophenyl)-2-Pyrazoline and Its Antibacterial Activity
.
International Conference on Chemistry, Chemical Process and Engineering (IC3PE)
AIP Conf. Proc.
1823
,
020051-1
020051-6
; doi:
3.
Amri
Setyawati
,
Tutik Dwi
W.
, and
Bambang
Purwono
2017 Synthesis of Novel Pyrazoline as Antibacterial Agents from Natural Product Vanillin
Asian Journal of Chemistry
Vol.
29
No.
2
454
456
.
4.
Khan
S.A.
,
Asiri
A.M.
,
Kumar
S.
and
Sharma
K.
Green synthesis 2014 antibacterial activity and computational study of pyrazoline and pyrimidine derivatives from 3-(3,4-dimethoxy -phenyl -1 - (2, 5 - dimethylthiophen-3-yl) - propenone
.
Eur J Chem.
5
(
1
):
85
90
.
5.
Shah
S.H.
,
Shukla
J.R.
and
Patel
P.S.
2014
Synthesis and BioEvaluation of Some New Phenyl Pyrazoline Derivatives from Pera-Chloro Benzaldehyde Moiety
.
J. Chem. & Cheml. Sci.
4
(
1
):
1
7
.
6.
Desai
P.R.
and
Desai
S.D.
2014
Studies on synthesis of biologically active pyrazoline derivatives
.
IRJC.
4
:
81
88
.
7.
Kumar
A.
,
Varadaraj
B.G.
and
Singla
R.K.
2013
Synthesis and evaluation of antioxidant activity of novel 3,5-disubstituted-2-pyrazolines.
Bulletin of Faculty of Pharmacy Cairo University
51
:
167
173
.
8.
Venkatesh
P.
,
Prasath
K.H.
,
Sharfudeen
S.
,
Soumya
V.
,
Spandana
V.
and
Priyanka
J.
2012
Synthesis of Coumarin fused Pyrazoline-5-one derivatives and Screening for their antimicrobial and antioxidant activity
Journal of Pharmacy Research
5
(
5
):
2875
2877
.
9.
Neethu
N.J.
and
Yusuf
S.
In-silico Design, Synthesis, Antiinflammatory and Anticancer A, Rozas I, Kelly B, Kaushik P, Kaushik D.
2014
Synthesis, Biological evaluation and Evaluation of Pyrazoline Analogues of Vanillin
Int. J. Pharm. Sci. Drug Res.
6
(
2
):
128
131
10.
Aggarwal
R.
and
Bansal
2013
Molecular modeling study of 5-trifluoromethyl-D2-pyrazoline and isomeric 5/3-trifluoromethylpyrazole derivatives as anti-inflammatory agents
.
Eur J Med Chem.
70
:
350
357
11.
Sridhar
S.
and
Rajendraprasad
Y.
2012
Synthesis and Analgesic Studies of Some New 2-Pyrazolines
.
E-Journal of Chemistry
9
(
4
):
1810
1815
.
12.
Wani
M.Y.
,
Bhat
A.R.
,
Azam
A.
,
Lee
D.H.
,
Choi
I.
and
Athar
F.
2012
Synthesis and in vitro evaluation of novel tetrazole embedded 1,3,5-trisubstituted pyrazoline derivatives as Entamoeba histolytica growth inhibitors
.
Eur J of Med Chem.
54
:
845
854
.
13.
Emayavaramban
M.
,
Santhi
N.
,
Gopi
C.
,
Manivannan
C.
and
Raguraman
A.
2013
Synthesis, Characterization and Antidiabetic activity of 1,3,5-triaryl-2-pyrazolines in acetic acid solution under Ultrasound Irradiation
.
International Letters of Chemistry, Physics and Astronomy
9
(
2
):
172
185
.
14.
Ovaisa
S.
,
Pushpalatha
H.
,
Reddy
G.B.
,
Rathore
P.
,
Bashira
R.
,
Yaseena
S.
,
Dheyaaa
A.
,
Yaseena
R.
,
Prakash
O.
,
Akthar
M.
,
Samima
M.
and
Javeda
K.
2014
Synthesis and biological evaluation of some new pyrazoline substituted benzenesulfonylurea/thiourea derivatives as antihyperglycaemic agents and aldose reductase inhibitors
.
Eur J Med Chem.
80
:
209
217
.
15.
Lange
J.H.M.
,
Attali
A.
,
Neut
MAWVD
,
Wals
H.C.
,
Mulder
A.
,
Zilaout
H.
,
Duursma
A.
,
Aken
HHMV
and
Vliet
BJV
.
2010
Two distinct classes of novel pyrazolinecarboxamides as potent cannabinoid CB1 receptor agonists
.
Bioorg. Med. Chem. Lett.
20
:
4992
4998
.
16.
Gupta
,
R.
,
Gupta
,
J.
, and
Jain
,
A.
2010
Improved synthesis of Chalcones and Pyrazolines Under Ultrasonic Irradiation
Indian J. Chem.
49B
351
355
.
17.
Hawaiz
,
F.E.
, and
Samad
,
M. K.
2012
Synthesis and Spectroscopic Characterization of Some New Biological Active Azo-Pyrazoline Derivatives
E. J. Chem.
9
(
3
),
1613
1622
.
18.
Sharshira
,
E.M.
, and
Hamada
,
N.M.M.
2011
Synthesis and In-Vitro Antimicrobial Activity of Some Pyrazolyl-1-carboxamide Derivatives
Molecules
16
7736
7745
.
19.
Fitzgerald
,
D.J.
,
Stratford
,
M.
,
Gasson
,
M.J.
,
Ueckert
,
J.
,
Bos
,
A.
, and
Narbad
,
A.
2004
Mode of Antimicrobial Action of Vanillin Against
Escherichia coli, Lactobacillus plantarum, and Listeria innocua J. App. Microbiol.
97
104
113
.
20.
Tortora
,
G.J.
,
Funke
,
B.R.
, and
Case
,
C.L.
2010
Microbiology an Introduction 10th Benjamin Cummings San Francisco.
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