Antibacterial activity of honey and lime fruit (Citrus aurantifolia (Christm.) Swingle) peeling waste extract against Escherichia coli and Staphylococcus aureus

Indonesia has a lot of potential natural source that can be used as a medicine, including limes fruit (Citrus aurantifolia (Christm.) Swingle) and honey. Lime fruit was a popular fruit consumed by public, but a bunch of peel waste leaves. Basically, fruit peeling waste of lime fruit has a big potential to be antibacterial agents, because it contains essential oils. On the other hand, the honey has been popular as a medicine, because of the presence of hydrogen peroxide, flavonoids, carotenoids, and phenol. This study aims to determine the antibacterial activity of fruit peel lime extract and some types of honey as well as a combination of both against Escherichia coli and Staphylococcus aureus. This study tried to formulate lime fruit peel extract using n-hexane with some types of honey. There are 13 kinds of antibacterial samples that will be tested on two types of bacteria, 1 single bark extract lime fruit, 6 single honey (KL21 honey, KP21 honey, R21 honey, Kelengkeng honey, Rambutan honey, and Trigona honey) and 6 combinations (KL21 honey-lime fruit extract, KP21 honey-lime fruit extract, R21 honey-lime fruit extract, Kelengkeng honey-lime fruit extract, Rambutan honey-lime fruit extract, and Trigona honey-lime fruit extract). The results showed that the samples of honey and n-hexane extract of lime peel have potential as a specific antibacterial. KP21 honey (25.20 ± 0.62) produces the greatest inhibition zone againts E. coli, whereas lime fruit extract (17.70 ± 0.98) produces the greatest inhibition zone againts S. aureus.