Assessment of the prenatal impact of manganese on behavioral responses and level of acetylcholinesterase in laboratory animals

The prenatal effect of manganese sulfate on the behavioral parameters of the body and the concentration of acetylcholinesterase in the blood serum was studied. For this purpose, female Wistar rats received manganese sulfate at a daily dose of 2/3LD₅₀ in addition to the main diet for 28 days before and during pregnancy (20-26 days). Young offspring were tested for the detection of behavioral disorders using standardized behavioral tests. According to the results of the tests, males with the greatest deviations were selected. The animals of this group were hyperactive. On reaching 18 weeks of age, blood was collected from the selected male rats to determine the level of acetylcholinesterase. The effect of oral exposure to manganese on the body of female mothers was also evaluated. The study showed that the serum of female rats exposed to direct exposure to manganese sulfate tended to have higher levels of acetylcholinesterase. Statistically significant changes in the level of this enzyme were observed in animals of the first generation: the Q₂₅ value of the first experimental group was more than the Q₇₅ of the group that was not exposed to prenatal exposure by 22% (p = 0.012). The study therefore demonstrated that prenatal exposure to manganese sulfate can affect the behavior and concentration of acetylcholinesterase in offspring.