Spectrophotometric determination of nystatin in its pharmaceutical preparations Available to Purchase

For spectrophotometric determination of Nystatin in its pure form and its pharmaceutical preparations, two simple, rapid ,and sensitive methods were developed. The proposed methods were: (I) synthesis of a Schiff’s base between Nystatin and Vanillin reagent in a basic medium to form a yellow-colored product, (II) while the second method depended on the oxidation of the Nystatin in the presence of acidic medium by a known excess of (Potassium bromide: Potassium bromate) (KBr: KBrO3) and subsequent determination of unreacted oxidant by reacting it with Crystal Violet to produce a blue product, the highest absorption was at 404 nm using Vanillin reagent and 592 nm when using Crystal violet dye. Obedience to Beer’s law occurred in the range of concentrations (5-100 µg/ml) and (2.5-25 µg/ml) with molar absorptivity 8.427×10³ L/mol.cm, and 2.472×10⁴ L/mol.cm for Vanillin and Crystal Violet, respectively, the limit of detection (LOD;) and limit of quantification were found to be 0.069 µg/ml and 0.213 µg/ml for Vanillin and Crystal Violet, respectively. Thus, the two methods are effectively quantify Nystatin pure form and in its pharmaceutical preparations.