This research article discussed about the role of microemulsion and TiO2 nanoadditives with Prosopis juliflora biodiesel which was blended and tested on the single cylinder diesel engine at various load conditions. The microemulsion and TiO2 nanoadditives produced better impact on the engine characteristics. The test fuel blends are diesel, Blend L (microemulsion+TiO2 (10%+20% Prosopis biodiesel+70% Diesel)), Blend T (20% Prosopis biodiesel+80% Diesel+25 ppm TiO2) and RAW OIL (100% Prosopis biodiesel) respectively. By using ultra-sonification process these test fuel blends were prepared and the diesel engine test result reveals better BTE and lowered rate of emissions at various load conditions.

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