Indirect spectrophotometric determination of cefotaxime using N-bromosuccinimide and crystal violet dye Available to Purchase

A spectrophotometric method is proposed to simply, rapidly, and precisely determine cefotaxime in pure and pharmaceutical formulation forms. The method is based on the oxidation of cefotaxime with N-bromosuccinamied in acidic medium of 1N H₂S0₄, the residual amount of oxidizing agent is then reacted with crystal violet dye. The maximum value of the absorbance of the dye is proportional to the amount of the determinants cefotaxime and achieved at 626 nm. Beer's law is obeyed in the concentration range of 1.2 – 36 µg/ml with a molar absorptivity of 1.266 Χ 10⁴ mol^-1.cm^-1, Sandell's sensitivity index of 0.36 µg.cm^-2, and relative standard deviation of 0.23 – 0.486 % depending on the concentration level. The limit of detection (LOD) and limit of quantification (LOQ) are 0.0591 and 0.1992 µg.ml^-1, respectively. The proposed method is successfully applied to determine cefotaxime in pure and pharmaceutical preparation.