Detection of chemical compounds and its antioxidant activity of aniseeds extract Available to Purchase

Anise is considered one of the economically and medically important plants that used by many peoples in the past, including Arab peoples, where has many compounds with useful properties. The aim of the current study was to detect the important chemical compounds in anise extract by hexane solvent using the GC-MS technique to show the types of compounds that have economic and medical importance for isolated from this plant in the future, in addition to the activity of antioxidation of this extract. Methods: In the experiment, plant extraction was prepared by used Soxhlet, were using hexane as a non-polar solvent, Then the compounds were detected by the GC-MS technique with the presence of additives Pyridine and MSTFA, also use DPPH and FRAP assays to examine the antioxidant activity of the extract. there was 25 chemical compounds, which were successively diagnosed according to the speed of their appearance depending on the retention time, Ethylphenyl acetate was found with the highest concentration among the compounds (26.988%), followed by Eugenol with a higher retention time and less concentration (16.509%), while Anethole was found with retention time higher than the two previous compounds, but less concentration (9.962%). Also found compounds not previously registered within the chemical content of the anise plant (Isobutyltrimethoxysilane Ethyltrimethoxysilane , Oxalic acid and Octatetracontane), which is due to the different conditions and the importance of using additives. As for the antioxidant efficacy, the high concentrations of anise extract showed great efficacy that was not significantly different from ascorbic acid, where (80, 90 and 100mg/ml) of anise extract showed (93.07%, 95.23% and 96.91%) in DPPH compare to (94.88%, 97.20% and 98.37%) for ascorbic acid respectively, also (90 and 100mg/ml) showed (0.301 and 0.390) in FRAP compare to (0.358 and 0.418) for ascorbic acid as control, and they were also close when compared with each other. As for the remaining concentrations, they showed weakness in their ability to scavenge roots OF DPPH and reduce of FRAP to show significant difference when compare to ascorbic acid. It was concluded from the current study that there are many compounds in the content of Anise plant that have high economic and medical importance that can be used for scientific and research purposes, in addition to the presence of new compounds that can be attributed to it the effectiveness of this plant. In addition, there is significant antioxidant activity of anise extract may be very important in the scientific field.