Histological changes in the liver, spleen and lung of mice in vitro experimentally infected with Crptococcus neoformans isolated from sputum with pulmonary tuberculosis

The study is case control done to investigate the histological alterations of the liver, spleen, and lung in the experimental male mice after trigger infection with Crptococcus neoformans microorganism which isolated from the sputum samples of patients returning to Tikrit Teaching Hospital who suffering from pulmonary tuberculosis from both genders, with ages ranged 15-82 yrs. The 63 samples were taken from patient who have candida infection and cultured on brain Infusion Agar/Broth to isolate the organism. This study included 4 groups of mice that expirmentallyinfected with the organism. Then a histological sample taken to observe the pathological tissue alteration in the liver, spleen and lung. The liver samples show presence of extensive degeneration in the hepatocytes, with notifying enlargement of hepatocyte and their nuclei, thickening in the nuclei and the appearance of numbers of cells in which the nuclei did not appear or appear ghostly. Coffer cells are presented in the hematologic sinuses between the hepatocytes, all the central veins branches of the portal vein are look like completely devoid of blood as no blood found in them, in compared to the control group. The spleen histological changes are severe necrosis of blood sinuses that appeared devoid of blood, infiltration of white blood cells. The histological changes in the lung show exfoliation of the columnar epithelial cells of the bronchi, heavy infiltration of the inflammatory white blood cells, severe blood congestion.