Anthocyanins are the compounds responsible for imparting dark colour to fruits and vegetables. The extraction of anthocyanin from Grapes (Vitis vinifera) skin by ultrasonic-assisted process was studied. Optimization of operating conditions, such as the ratio of amplitude, extraction temperature and extraction time, on the extraction yield of anthocyanins was carried out through response surface methodology (RSM). The developed experimental design with a factorial 33, where the factors were amplitude, temperature, time at the levels 20%, 40%, 60%, at times 5 min, 10 min, 25 min, and at temperatures 20°C, 30°C, and 50°C for each factor respectively. Using response surface methodology, the optimal set of extraction variables were obtained for maximizing the concentration of anthocyanins from the grape peel extracts. Ultrasonic- assisted extraction is found to be more efficient and rapid for extraction. Having higher amplitude at 60% achieved maximum extraction levels (300.4 – 304.5 mg). Reduction in extraction volume was comparable at such amplitude and extraction time for temperatures 20 to 40 °C. Allowing 60 min extraction time at 60% amplitude achieved maximum extraction levels at 50 °C which is comparable to theresultspredicted by the statistical tool as 60%, 44°C for 20 min.

1.
Sánchez-Alonso
,
Isabel
,
Antonio
Jiménez-Escrig
,
F.
Saura-Calixto
, and
A. Javier
Borderías
Antioxidant protection of white grape pomace on restructured fish products during frozen storage
LWT-Food science and Technology, vol.
41
, no.
1
,
2008
, pp.
42
50
.
2.
Meyer,
Anne
S.
,
Ock-Sook
Yi
,
Debra A.
Pearson
,
Andrew L.
Waterhouse
, and
Edwin N.
Frankel
Inhibition of human low-density lipoprotein oxidation in relation to composition of phenolic antioxidants in grapes (Vitis vinifera
)”
Journal of Agricultural and Food Chemistry, vol.
45
, no.
5
,
1997
, pp.
1638
1643
.
3.
Pinelo
,
Manuel
,
Anis
Arnous
, and
Anne S.
Meyer
Upgrading of grape skins: Significance of plant cell-wall structural components and extraction techniques for phenol release
Trends in Food Science & Technology
, vol.
17
, no.
11
,
2006
, pp.
579
590
.
4.
Carrera
,
Ceferino
,
Ana
Ruiz-Rodríguez
,
Miguel
Palma
, and
Carmelo G.
Barroso
Ultrasound assisted extraction of phenolic compounds from grapes
Analytica chimica acta
, vol.
732
,
2012
, pp.
100
104
.
5.
Amrani-Joutei
,
K.
, and
Y.
Glories
Tannins and anthocyanins of grape berries: Localization and extraction technique
Rev. Fr. Oenol,
vol.
153
,
1995
, pp.
28
31
.
6.
Lee
,
Jungmin
,
Robert W.
Durst
,
Ronald E.
Wrolstad
, and Collaborators:
Eisele
T
Giusti
MM
Hach
J
Hofsommer
H
Koswig
S
Krueger
DA
Kupina
;
S
Martin
SK
Martinsen
BK
Miller
TC
Paquette
F
RyabkovaASkrede
G
Trenn
U
Wightman
JD
Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study
Journal of AOAC international,
vol.
88
, no.
5
,
2005
, pp.
1269
1278
.
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