This study was intended to study the biological activity of probiotic against MOR bacteria isolated from burns infection. Seventy-Five samples and inquiries were carried out between October 2020 and March 2021. Burn infection samples were obtained from two separate hospitals in the province of Najaf (AL Sadder Medical City and AL Forat hospital). Isolates were identified based on the morphological and microscopic examination, and VITEC. The results showed that the most common Gram-positive was S. aureus (24%), while Gram-negative bacilli were the most common P. aeruginosa (53%) followed by Klebsiella pneumoniae (13%), Escherichia coli (6%) Proteus mirabilis (2.6%). Molecular study was detected the antibiotic resistance genes blaTEM-1, GES and bla-OXA in P. aeruginosa, K. pneumoniae, E. coli and P. mirabilis using conventional PCR. one efficient isolate(Z2) was selected from screening different strains (25) of probiotics based on antibacterial activity. Isolate Z2 was identified as Bacillus subtilis depending on morphology, microscopic examination, and VITEK. The study showed the inhibitory ability of B. subtilis against all tested isolate as well as Bacillus subtilis showed high anti-biofilm activity against studied bacteria.

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