Three potential Bacillus sp. strain, KRG, KRT, and LDR from cocopeat was known to have antagonistic activity against fungal pathogen. The antagonistic activity represented the potency of three isolates as biocontrol agent. In this study, the growth and extracellular enzyme of three isolates will be characterized and also the selected bacterium was identified based on 16S rRNA. The growth of Bacillus sp. strains were characterized in Potato Dextrose Broth (PDB) with various pH medium and temperature incubation. The three isolates can growth at 30–45 °C, meanwhile KRG can grew up to 50 °C. The optimum temperature growth of LDR was 30–45 °C. All of isolates can grow in medium with pH 5–8 with optimal growth for LDR in pH 7–8. Extracellular enzyme assay showed all isolates can produce amylase, lipase, protease, and gelatinase. Molecular identification showed that selected strain LDR is closed to Bacillus siamensis and has 99.52 % similarity with Bacillus siamensis KCTC 13613 strain PD-A10.
Skip Nav Destination
Research Article| July 23 2021
Characterization of potential biocontrol agent Bacillus sp.
I. Santoso a)
Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas IndonesiaDepok 16424,
Center of Excellence for Indigenous Biological Resources-Genome Studies (CoE IBR-GS), Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok, 16424
Search for other works by this author on:
Q. G. Fadhilah;
A. E. Maryanto;
AIP Conf. Proc. 2374, 050002 (2021)
I. Santoso, Q. G. Fadhilah, A. E. Maryanto, Yasman; Characterization of potential biocontrol agent Bacillus sp.. AIP Conf. Proc. 23 July 2021; 2374 (1): 050002. https://doi.org/10.1063/5.0058783
Download citation file:
Don't already have an account? Register
You could not be signed in. Please check your credentials and make sure you have an active account and try again.
Could not validate captcha. Please try again.