Chikungunya virus infection (CHIKV) causes symptoms of chikungunya fever and joint pain. The chikungunya virus is spread by the bite of an Aedes mosquito. The symptoms of CHIKV and dengue virus (DENV) infection are similar and are spread by the same vector. Diagnosis of CHIKV infection is carried out by expensive molecular detection and immunological detection (RDT) as an alternative diagnosis. The material to be used for the development of RDT CHIKV is the envelope 2 protein (E2) CHIKV. This study aims to obtain pPICZaA-E2 which is transformed into Escherichia coli TOP10. The pPICZaA plasmid and the E2 CHIKV gene were cloned into E. coli TOP10 and grown onto LB+zeocin agar medium. Cultures grown on the medium were verified for colonies carrying pPICZaA-E2 using PCR colony and restriction. The PCR verification results of the colonies from the growing cultures showed a band measuring 1.260 bp. The results of the restriction verification obtained colonies with two bands measuring 3.569 and 1.260 bp. It was concluded that the E. coli TOP10 colonies carried pPICZaA-E2. Sequencing of the isolated pPICZaA-E2 plasmid is required.
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23 July 2021
PROCEEDINGS OF THE 6TH INTERNATIONAL SYMPOSIUM ON CURRENT PROGRESS IN MATHEMATICS AND SCIENCES 2020 (ISCPMS 2020)
27–28 October 2020
Depok, Indonesia
Research Article|
July 23 2021
Cloning of chikungunya virus envelope 2 (E2) gene to pPICZaA in Escherichia coli TOP10
F. A. Sitepu;
F. A. Sitepu
1
Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia
, Depok 16424, Indonesia
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S. Pambudi;
S. Pambudi
2
Center of Pharmaceutical and Medical Technology, Agency for the Assessment and Application of Technology (BPPT)
, Puspiptek - Serpong, Tangerang Selatan 15310, Indonesia
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F. Shabihah;
F. Shabihah
1
Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia
, Depok 16424, Indonesia
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C. Ikhsan;
C. Ikhsan
1
Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia
, Depok 16424, Indonesia
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B. Yohan;
B. Yohan
3
Eijkman Institute for Molecular Biology, Ministry of Research and Technology
, Jl. Diponegoro 69, Jakarta 10430, Indonesia
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R. Lestari
R. Lestari
a)
1
Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia
, Depok 16424, Indonesia
a)Corresponding author: retno.lestari@sci.ui.ac.id
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a)Corresponding author: retno.lestari@sci.ui.ac.id
AIP Conf. Proc. 2374, 050001 (2021)
Citation
F. A. Sitepu, S. Pambudi, F. Shabihah, C. Ikhsan, B. Yohan, R. Lestari; Cloning of chikungunya virus envelope 2 (E2) gene to pPICZaA in Escherichia coli TOP10. AIP Conf. Proc. 23 July 2021; 2374 (1): 050001. https://doi.org/10.1063/5.0059272
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