Kopasanda (Chromolaena odorata (L.) R.M. Rob, Asteraceae) is a, distributed in the tropical, and subtropical, countries including Indonesia. C. odorata have been reported to show biological and pharmacological activities. This work purposed to determine the profile and composition of polyprenols and dolichols from C. odorata leaves senescence (bud, young, and old leaves), investigated by two-dimensional thin layer chromatography (2D-TLC) technique. In these tissues, one-type, type-II of a profile of presence polyprenols and dolichols was observed. In contrast, Type-I and Type-III was not detected. In the buds, the shorter carbon chain length and the longer one belongs to old leaves with a carbon chain length of polyprenol C70-C95 and dolichol of C70-C95. The pattern of polyisoprenoids in this senescence leaves stage indicated the chemotaxonomic significance of polyisoprenoids in this species. The current report confirmed the occurrence of polyisoprenoids in kopasanda buds, young and old leaves.

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