Indonesia is the second country producing Cassava (Manihotesculenta Crantz) after Nigeria. One of the Indonesian Province, Lampung, is a Cassava central land in Indonesia, with a total production of 7,39 million tons in 2015. However, there are still many Cassava cultivation production constraints, such as Fusarium wilt disease. The disease is caused by the fungus Fusariumoxysporum, which until now still cannot be effectively addressed. So far, the farmers have eradicated the fungus by using fungicides, which have adverse impacts on the environment. Therefore, it's necessary to control the disease biologically by using a superior variety resistant to Fusariumoxysporum. Fusaric acid is a toxin produced by Fusariumoxysporum fungus which is mostly found in vitro selection in plants. Disease resistance can be obtained by induced resistance. The purpose of this study is to analyze the content of the reducing sugar of the Cassava plantlet after induced fusaric acid in vitro. The research using Completely Randomized Design, of one factor, with fusaric acid concentrations, consists of 5 levels 0 ppm, 60 ppm, 80 ppm, 100 ppm, dan 120 ppm in Murashige and Skoog medium. The data analysis used Analysis of Variance with a 5 percent significance level. The results of this study indicate that the increased concentration of fusaric acid, then also increases the reducing sugar content in the Fusariumoxysporum resistant Cassava plantlet.

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