Gel electrophoresis: The applications and its improvement with nuclear technology Available to Purchase

Gel electrophoresis is a standardized technique that is routinely used in many daily needs, such as clinical laboratories, forensic and industry, molecular biology and biochemistry, conservational biology, and medicine. This paper reviews several types of techniques, with two mostly used technique being agarose and acrylamide gel electrophoresis, either isotopic or non-isotopic based analysis. This technique exploits the difference in size and charge of various molecules in the sample being analyzed. An example of the clinical application of gel electrophoresis is to detect the presence of quantitatively or qualitatively abnormal protein bands. Alterations in the relative concentration of sample fractions allow easy recognition of pathological disorders associated with a disease. In the food industry, it is applied to safeguard the quality of food products. The advantages of this technique are also described that are simple, sensitive, short analysis time, low cost, and environment friendly. The range of its application and modification continues to expand. Here, an application of radioisotope labeled electrophoresis in the analysis of 50 drug resistance of tuberculosis bacteria had been done and showed that several samples had altered its DNA mobility shift on the gel that related to specific gene mutation. Nuclear based analysis has shown improvement as it is relatively more sensitive compared to conventional one but high risk. It can be concluded that gel electrophoresis has proven to be one of the most useful and versatile techniques in biological sciences research.