The problem to which the claimed study is to improve the methods of immunostaining of large biological objects, including mammalian embryos, isolated whole organs, and marine invertebrates through the use of penetrating agents and detergents for the delivery of antibodies and their subsequent visualization after chemical clearance. The advantages of the proposed method are adaptability for different biological objects. This method allows to easily visualizing the deeper tissue structures of organs, embryos of vertebrates, and also, for the first time, the method is adapted for invertebrate animals. The method involves the use of DMSO penetrating agents, solutions of blocking and incubation with antibodies and chemical enlightenment using a mixture of benzyl alcohol and benzyl benzoate.

1.
K.
Tainaka
,
A.
Kuno
,
S. I.
Kubota
,
T.
Murakami
, and
H.R.
Ueda
.
Annu. Rev. Cell Dev. Biol.
32
,
713
41
(
2016
).
2.
M.N.
Economo
,
N.G.
Clack
,
L.D.
Lavis
,
C.R.
Gerfen
,
K.
Svoboda
.
A platform for brain-wide imaging and reconstruction of individual neurons
.
eLife
5
:
e10566
(
2016
).
3.
K.L.
Ode
,
H.R.
Ueda
.
Diabetes Obes. Metab.
17
,
47
54
(
2015
).
4.
S.W.
Oh
,
J.A.
Harris
,
L.
Ng
,
B.
Winslow
,
N.
Cain
.
Nature
508
,
207
14
(
2014
).
5.
T.
Ragan
,
L.R.
Kadiri
,
K.U.
Venkataraju
,
K.
Bahlmann
,
J. Sutin. Nat. Methods
9
,
255
58
(
2012
).
This content is only available via PDF.
You do not currently have access to this content.