Three-dimensional (3D) porous films based on chitosan (CS)/carboxymethyl guar gum (CMGG) polyelectrolyte complex (PEC) containing titanium dioxide nanoparticles (TiO2 NPs) were prepared as a wound-healing material by the freeze-drying process. The physicochemical properties of these films were evaluated by FTIR, XRD, SEM and EDX analysis. Furthermore, the swelling degree, porosity, in vitro biodegradation and compression strength of these films were examined. The developed films showed the biocompatibility against the C3H10 T1/2 cells and antimicrobial activity towards gram-positive and gram-negative bacteria and fungi. The CS/CMGG/TiO2 films with 2 wt. % of TiO2 NPs exhibited the improved cell migration rate compared to the control CS/CMGG film. These findings indicated that the porous CS/CMGG/TiO2 films could have the potential to be used as a wound-healing material.

Topics
Materials degradation, Natural materials, Porous media, Nanoparticle, Antimicrobials, Bacteria, Fungi, Biomaterials, Carbohydrates, Cell migration
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