Zinc oxide (ZnO) nanoparticles were synthesized by thermal decomposition method using zinc carbonate as precursor and potassium hydroxide as starting materials. These particles were characterized by using UV visible spectrophotometer, thermoluminescence (TL) reader. The biological function of ZnO nanoparticle was tested against the Pseudomonas aeruginosa, Candidaalbicans and Escherichia colibacteria. UV absorption peak observed at 353 nm and suggested an energy band gap of 3.51 eV for the compound under study.TL curve indicates the electron trapping site in ZnO nanostructures with a very large band gap.A significant antibacterial activity was observed in case of Pseudomonasaeruginosa, which indicates that the ZnO nanoparticles have greatest antimicrobial activity against Pseudomonasaeruginosa.

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