The catalyst of banana stem ash was used in the reaction of biodiesel formation with acetone as a co-solvent transesterification reaction. This research aimed to study the character and content of the catalyst and the effect of the mass amount of the catalyst of banana stem ash on the results of the transesterification reaction of waste cooking oil at room temperature for 20 minutes. The mole ratio of used cooking oil in methanol is 1:12. The percentage of the addition of the catalyst is 1%, 3%, and 5%, respectively. Heating catalyst ash at a temperature of 750°C was characterized using XRD. The result shows the phase of the oxide metals from alkali and transition. The results of the transesterification reaction were analyzed using the GC-MS instrument. The results showed that with a 5% catalyst the yield was 80.31%. The implication of this research is that it can multiply low-temperature biodiesel production by utilizing banana waste as a catalyst so that biodiesel production becomes efficient and cheaper.

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