The aim of this work is to optimize EPD variables (voltage, time and concentration) using DC current depends on thickness and roughness and then characterization coating layer to select optimum condition. Electrophoresis deposition (EPD) attracts a great deal of attention to the treatment of biological materials, especially coatings in biomedical and nanomaterials as well as collections of unique and complex materials, focusing on the great smell of bone culture. The hydroxyapatite powder deposition by electrophoretic was performed on Ti-6Al-4V alloy by utilizing ethanol as a solvent under DC, to enhance the quality of the surface alloy and to meet the requirements the activity of the biological orthopedic application. The approach of Taguchi was utilized in order to determine the optimal conditions of the coatings deposition. various parameters were applied to deposit the biochemical coatings (time, concentration and voltage of powders). Solvents were used to precipitate chitosan with hydroxyapatite to be ethanol at pH 4. The surface morphology and the cross section of the coating were characterized by optical microscopes, XRD, and SEM. Optimal conditions for precipitation of hydroxyapatite (HAP) layers using roughness and thickness of 20 volts, 6 minutes, 3g / l, 40 volts, 4 min and 1 g / l on presence at room temperature. The water contact angles of samples are changed between 67.489 ° C and 35.914° C. Appropriate proportions of porosity were obtained with an average volume of 5.05-85.47 µm were obtained.

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