In this research, we have synthesized silver nanoparticles with a pulsed laser using a metallic silver (purity; 99.99%), the targeted metallic silver piece was submerged in a liquid containing Polyvinylpyrrolidone (PVP). The produced nanoparticles were observed with different methods included UV-Vis Spectrometer, Atomic Absorption (AAS) and Electron Microscope (TEM). All peak absorption spectra for the two types of nanoparticles were ranged between 401 and 411 nm whereas nanoparticles size were in the range between 1 and 70 nm. The malignant anti-proliferation activity of nanoparticles was tested on murine mammary gland cancer cell line (AMN3). This activity was with potent inhibitory effect on the cellular proliferation rate of the cells under investigation. The inhibitory action was elevated when both the concentration of silver nanoparticles (AgNPs) and time of exposure were increased in comparison with the control untreated cells. Staining the cells with crystal violet stain revealed an increase in the level of cellular destruction. The results demonstrated that exposing the malignant cells to the nanomaterial of silver increases the proportion of the cells that undergo mitochondrial membrane disruption and DNA fragmentation and that will result in the induction of apoptosis. These results confirm that PVP-coated AgNPs induces apoptosis and cancer cell death.

1.
H.F.
Krug
,
P.
Wick
,
Angewandte Chemie International Edition
,
50
,
1260
78
(
2011
).
2.
S.D.
Caruthers
,
S.A.
Wickline
,
G.M.
Lanza
,
Curr Opin Biotechnol
,
18
,
26
30
(
2007
).
3.
P.
Devaraj
,
C.
Aarti
,
P.
Kumari
,
Int J Pharm Pharm Sci
,
6
,
86
90
(
2014
).
4.
S.
Priyaragini
,
S.R.
Sathishkumar
,
K.V.
Bhaskararao
,
Int J Pharm Pharm Sci
,
5
,
709
12
(
2013
).
5.
Z.Q.
Ji
,
H.
Sun
,
H.
Wang
,
Q.
Xie
,
Y.
Liu
,
Z.
Wang
.
J Nanopart Res
,
8
,
53
63
(
2006
).
6.
S.
Dugal
,
S.
Chakraborty
,
Int J Pharm Pharm Sci
5
,
498
501
(
2013
).
8.
O.
Salata
,
J Nanobiotechnol
2
,
1
6
(
2004
).
9.
J.
Chen
,
M.K.
Choe
,
S.
Chen
,
S.
Zhang
,
AIDS Educ Prev
17
,
1
11
(
2005
).
10.
J.L.
Elechiguerra
,
J.L.
Burt
,
J.R.
Morones
,
A.
Camacho-Bragado
,
X.
Gao
,
H.H.
Lara
J Nanobiotechnol
3
,
6
(
2005
).
11.
B.
Nowack
,
H.F.
Krug
,
M
,
Height
,
Environ Sci Technol
45
,
1177
83
(
2011
).
12.
S.R.
Johnston
,
Anticancer Drugs.
8
,
911
930
(
1997
).
13.
S.
Pervin
,
A.H.
Tran
,
S.
Zekavati
,
J.M.
Fulknto
,
R.
Singh
,
G.
Chaudhuri
,
Cancer Res.
68
,
4862
4874
(
2008
).
14.
L.Z.
Flores-López
,
H.
Espinoza-Gómez
,
R.
Somanathan
,
Journal of Applied Toxicology
39
,
16
26
(
2019
).
15.
A.T.
Tawfeeq
,
Iraqi Journal of Cancer and Medical Genetics
,
7
,
69
76
(
2014
).
16.
X.F.
Zhang
,
Z.G.
Liu
,
W.
Shen
, and
S.
Gurunathan
,
International journal of molecular sciences
,
17
,
1534
(
2016
).
17.
L.
Florento
,
R.
Matias
,
E.
Tuaño
,
K.
Santiago
,
F. dela
Cruz
,
amd A.
Tuazon
,
International journal of biomedical science: IJBS
,
8
,
76
(
2012
).
18.
Y.
Ren
,
G.D.
Anaya-Eugenio
,
A.A.
Czarnecki
,
T.N.
Ninh
,
C.
Yuan
,
H.B.
Chai
,
A.D.
Kinghorn
,
Bioorganic & medicinal chemistry
,
26
,
4452
4460
(
2018
).
19.
K.B.
Ng
,
A.
Bustamam
,
M.A.
Sukari
,
S.I.
Abdelwahab
,
S.
Mohan
,
M.J.C.
Buckle
,
H.S.
Rahman
,
BMC complementary and alternative medicine
,
13
,
41
(
2013
).
20.
S.
Samarghandian
, and
M.M.
Shabestari
,
Indian journal of urology: IJU: journal of the Urological Society of India
,
29
,
177
(
2013
).
21.
R.
Zamiri
,
B.Z.
Azmi
,
H.A.
Ahangar
,
G.
Zamir
,
M.S.
Husin
, and
Z.A.
Wahab
,
Bull. Mater. Sci.
35
,
727
731
(
2012
).
22.
N.N.R.
Alrawi
,
M.Q.
Al-Ani
, and
N.Y.
Yaseen
,
Asian J Pharm Clin Res
,
11
,
375
382
(
2018
).
23.
F.
Di Nicolantonio
,
S.J.
Mercer
,
L.A.
Knight
,
F.G.
Gabriel
,
P.A.
Whitehouse
,
S.
Sharma
,
S.S.
Somers
,
BMC cancer
,
5
,
78
(
2005
).
24.
P.V.
AshaRani
,
G. Low Kah
Mun
,
M.P.
Hande
, and
S.
Valiyaveettil
,
ACS nano
,
3
,
279
290
(
2008
).
25.
C.
Völker
,
M.
Oetken
, and
J.
Oehlmann
, “The biological effects and possible modes of action of nanosilver. In
Reviews of Environmental Contamination and Toxicology
” (
New York
:
Springer
,
2013
), pp.
81
106
.
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