Novel approach of junction cell for on – Line fast chemiluminescence reaction and light energy transfer by 8 mm optical fibers

Folic acid was determined by a new, accurate, sensitive and rapid indirect method through an adduct derivative formation of folic acid via continuous mode of FIA coupled with a homemade of a new design of Chemiluminescence cell for measurement of the chemiluminescence signal that results from the reaction of luminol molecule in alkaline medium with folic acid to produce of an adduct derivative of luminol-folic acid. Under the optimum established conditions, the linear range of 0.0001-0.14 mmol L⁻¹ along with correlation coefficient (r) of 0.9967, Limit of detection (LOD). (S/N =3) 1.545 ng / 70 µL were achieved. Precision expressed as relative standard deviation for eight replicates measurements using 0.1 mmol.L⁻¹ folic acid; less than 1% were obtained for folic acid. The newly developed methodology was successfully applied for the estimation of folic acid in two drugs of different companies. The comparison that was made between two methods by the use of standard addition method and applied: individual t-Test and paired t-Test. It shows, there was no differences between the claimed values of each difference individual company with t calculated -value at, 95 %, confidence level. In addition to there were no significant differences between the two methods.