The phenomenon of smoking starts to increase very fast around the world. This increase in smoking comes with the increase of use of the different kinds of tobacco among the people who are smoking in Iraq. The goal of this research wasto assesstheconnection and interactionof genotypic polymorphism in methylenetetrahydrofolate reductase genein C677T position with different type of smoking. The Methods two distinct test tubes used to gathered venous blood samples from each topic, one was used for biochemical analysis and the other EDTA tube used for DNA extraction. From all samples patients and control subjects the Genomic DNA was isolated from blood. By Bio drop DNA concentration and purity were determined measuring done, and validate the concentration of DNA for PCR reaction. By using agarose gel electrophoresis the quality of the DNA was determined and stained with Red safe DNA, at -20 °C all samples were stored until further utilization. The current study showed increase hematological test and biochemical parameters in smokers compare with control subject. In this study about the polymorphism ofMTHRF/C677T gene, the results show that there is a mutation C266T in all groups of study, but in different percentage, the highest percentage for the Genotype TT is 30 % for those who smoke both Hokka and cigarette. While the Genotype CT for those who just smoke Hokka is considered the highest one 20%, and the percentage for Genotype CT and TT for those who just smoke cigarette is considered the lowest one which is just 10% for each Genotype. In this study, MTHFR gene polymorphism associated with different type of smoking. In addition, we found an association between the MTHFR C677T polymorphism variant genotype (TT) and increased risk disorders in smokers.

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