Conifer is one of the most important medicinal plants distributed around the world. These trees are widely distributed in the north of Iraq. Among these conifers which presented in Iraq is Cupressussempervirens (cypress). This plant was reported to contain many active constituents.Cones of cypress were divided into two parts, one was extracted with ethanol while the other extracted with hexane and then the mark was re-extracted with aceton/water.Phytochemical investigation was performed using chemical reactions.Antimicrobial activity of cone extracts is studied using agar well diffusion method. DPPH assay is used for detecting the antioxidant activity.The phytochemical analysis of ethanolic extract of cone of C.sempervirens revealed the presence of tannin, glycoside, alkaloid, terpene and flvonoid, cone extractsshowed antimicrobial and antioxidant activity as compared with ciprofloxacin and ascorbic acid respectively, ethanolic extract showed the best result in both cases.Different type of cone extracts of C. sempervirens showed antimicrobial and antioxidant activity, both are important in the treatment of many disease so further study are needed to investigate phytochemical component of other parts of the plant and to study the biological activity of these compounds.

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