Phenolic antioxidants BHA and BHT are commonly used synthetic antioxidants. It is commonly used in edible oils, oils and fats, and plastic packaging materials to increase the service life of plastic packaging materials to prevent oxidation and decomposition of materials, thereby protecting edible oils or plastics from oxidative damage. However, during long-term contact with plastic materials or products, antioxidants can also migrate into food through different channels, thus endangering consumers’ health. In order to keep abreast of usage and ensure consumer safety, it is important to develop rapid detection techniques for these two substances. Therefore, this study established an HPLC method for simultaneously detecting fat-soluble antioxidants BHA and BHT in foods and pharmaceuticals. This method optimizes detector wavelength, mobile phase conditions, and other chromatographic parameters. In this study, Waters XBridge C18 was used as the analytical column to compare the gradient elution effects of various mobile phases, and finally use methanol and acetonitrile as the gradient eluted mobile phase. BHA and BHT have a good linear relationship in the range of 2.0-100 ug/ml (r2>0.999). The method is convenient in operation and high in sensitivity, and can be used for rapid detection of antioxidants in food and pharmaceutical packaging materials.

1.
Tormin
T. F.
,
Cunha
R. R.
,
Richter
E. M.
, et al. 
Fast simultaneous determination of BHA and TBHQ antioxidants in biodiesel by batch injection analysis using pulsed-amperometric detection
[J].
Talanta
,
2012
,
99
:
527
531
.
2.
Ding
M.
,
Zou
J.
Rapid micropreparation procedure for the gas chromatographic–mass spectrometric determination of BHT, BHA and TBHQ in edible oils
[J].
Food chemistry
,
2012
,
131
(
3
):
1051
1055
.
3.
Medeiros
R. A.
,
Rocha-Filho
R. C.
,
Fatibello-Filho
O.
Simultaneous voltammetric determination of phenolic antioxidants in food using a boron-doped diamond electrode
[J].
Food chemistry
,
2010
,
123
(
3
):
886
891
.
4.
Medeiros
R. A.
,
Lourenção
B. C.
,
Rocha-Filho
R. C.
, et al. 
Simple flow injection analysis system for simultaneous determination of phenolic antioxidants with multiple pulse amperometric detection at a boron-doped diamond electrode
[J].
Analytical chemistry
,
2010
,
82
(
20
):
8658
8663
.
5.
Galimany-Rovira
F.
,
Pérez-Lozano
P.
,
García-Montoya
E.
, et al. 
Optimization and validation of a fast UPLC method for simultaneous determination of hydroquinone, kojic acid, octinoxate, avobenzone, BHA, and BHT
[J].
Journal of AOAC International
,
2017
,
100
(
1
):
1
7
.
6.
Caramit
R. P.
,
de Freitas Andrade
A. G.
,
de Souza
J. B. G.
, et al. 
A new voltammetric method for the simultaneous determination of the antioxidants TBHQ and BHA in biodiesel using multi-walled carbon nanotube screen-printed electrodes
[J].
Fuel
,
2013
,
105
:
306
313
.
7.
Freitas
K. H. G.
,
Fatibello-Filho
O.
Simultaneous determination of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) in food samples using a carbon composite electrode modified with Cu3 (PO4) 2 immobilized in polyester resin
[J].
Talanta
,
2010
,
81
(
3
):
1102
1108
.
8.
Medeiros
R. A.
,
Rocha-Filho
R. C.
,
Fatibello-Filho
O.
Simultaneous voltammetric determination of phenolic antioxidants in food using a boron-doped diamond electrode
[J].
Food chemistry
,
2010
,
123
(
3
):
886
891
.
9.
Galimany-Rovira
F.
,
Pérez-Lozano
P.
,
Suñé-Negre
J. M.
, et al. 
Development and validation of a new RP-HPLC method for the simultaneous determination of hydroquinone, kojic acid, octinoxate, avobenzone, BHA and BHT in skin-whitening cream
[J].
Analytical Methods
,
2016
,
8
(
5
):
1170
1180
.
This content is only available via PDF.
You do not currently have access to this content.