Establishment of rapid detection method for fat-soluble antioxidants BHA and BHT

Phenolic antioxidants BHA and BHT are commonly used synthetic antioxidants. It is commonly used in edible oils, oils and fats, and plastic packaging materials to increase the service life of plastic packaging materials to prevent oxidation and decomposition of materials, thereby protecting edible oils or plastics from oxidative damage. However, during long-term contact with plastic materials or products, antioxidants can also migrate into food through different channels, thus endangering consumers’ health. In order to keep abreast of usage and ensure consumer safety, it is important to develop rapid detection techniques for these two substances. Therefore, this study established an HPLC method for simultaneously detecting fat-soluble antioxidants BHA and BHT in foods and pharmaceuticals. This method optimizes detector wavelength, mobile phase conditions, and other chromatographic parameters. In this study, Waters XBridge C18 was used as the analytical column to compare the gradient elution effects of various mobile phases, and finally use methanol and acetonitrile as the gradient eluted mobile phase. BHA and BHT have a good linear relationship in the range of 2.0-100 ug/ml (r2>0.999). The method is convenient in operation and high in sensitivity, and can be used for rapid detection of antioxidants in food and pharmaceutical packaging materials.