Taking the wine grape ‘Cabernet Sauvignon’ as test material, the effect of 50 mmol·L−1 NaCl spraying treatment on ABA synthesis and hardness of grape berry in veraison was investigated. The influence of NaCl and water on the initiation of fruit ripening and the formation of fruit quality were studied. The results showed that the hardness of salt-treated berry was higher than control in veraison. Additionally, compared with control group, the accumulation rate of anthocyanin and soluble solids in berry were both significantly reduced under NaCl treatment during the development process. While the content of titratable acidity showed an inverse change with increasing the content of tartaric acid and malic acid. Furthermore, the ABA synthesis of grape skin and flesh was delayed under salt stress, which decreased the accumulation rate of ABA accumulation. Quantitative real-time PCR analysis showed that VvNCEDs, the key gene of ABA synthesis, was down-regulated under salt stress, which was consistent with the change of ABA content.
REFERENCES
1.
F. M. D.
Amor
, V.
Martinez
, A.
Cerda
. Salinity Duration and Concentration Affect Fruit Yield and Quality, and Growth and Mineral Composition of Melon Plants Grown in Perlite
[J]. H ortscience A Publication of the American Society for Horticultural Science
, 1999
, 34
(7
): 1234
–1237
.2.
P.
Botia
, J. M.
Navarro
, A.
Cerda
, et al Yield and fruit quality of two melon cultivars irrigated with saline water at different stages of development
[J]. European Journal of Agronomy
, 2005
, 23
(3
): 243
–253
.3.
A. M.
Grieve
, L. D.
Prior
, K. B.
Bevington
. Long-term effects of saline irrigation water on growth, yield, and fruit quality of ‘Valencia’ orange trees
[J]. Australian Journal of Agricultural Research
, 2007
, 58
(4
): 342
–348
.4.
Y.
Dumas
, M.
Dadomo
, D. G.
Lucca
, et al Effects of environmental factors and agricultural techniques on antioxidantcontent of tomatoes
[J]. Journal of the Science of Food & Agriculture
, 2003
, 83
(5
): 369
–382
.5.
S.
Krauss
, W. H.
Schnitzler
, J.
Grassmann
, et al The influence of different electrical conductivity values in a simplified recirculating soilless system on inner and outer fruit quality characteristics of tomato
[J]. J Agric Food Chem
, 2006
, 54
(2
): 441
–448
.6.
H.
Gautier
, F.
Lopez-lauri
, C.
Massot
, et al Impact of ripening and salinity on tomato fruit ascorbate content and enzymatic activities related to ascorbate recycling
[J]. Function Plant Science and Biotechnology
, 2010
, 4
: 66
–75
.7.
S. W.
Lu
, T. L.
Li
, J.
Jing
. Effects of salinity on sucrose metabolism during tomato fruit development
.[J]. African Journal of Biotechnology
, 2010
, 9
(9
): 842
–849
.8.
Li
X. L.
, Wang
C. R.
, Li
X. Y.
, et al Modifications of Kyoho grape berry quality under long-term NaCl treatment
[J]. Food Chemistry
, 2013
, 139
(1-4
):931
–937
.9.
Hong
Sun
, Tianyu
Sun
, Lili
Xu
, et al Effects of long-term low-salt treatment on grape fruit quality and transcriptome
[J]. Plant Physiology Journal
, 2017
(12
): 2197
–2205
.10.
S. D.
Castellarin
, G. A.
Gambetta
, H.
Wada
, et al Characterization of major ripening events during softening in grape: turgor, sugar accumulation, abscisic acid metabolism, colour development, and their relationship with growth
[J]. Journal of Experimental Botany
, 2016
, 67
(3
): 709
–722
.11.
T. R.
Thomas
, K. A.
Shackel
, M. A.
Matthews
. Mesocarp cell turgor in Vitis vinifera L. berries throughout development and its relation to firmness, growth, and the onset of ripening
[J]. Planta
, 2008
, 228
(6
): 1067
–1076
.12.
G. A.
Gambetta
, M. A.
Matthews
, T. H.
Shaghasi
, et al Sugar and abscisic acid signaling orthologs are activated at the onset of ripening in grape
[J]. Planta
, 2010
, 232
(1
): 219
–234
.13.
Jia
H. F.
, Chai
Y. M.
, Li
C. L.
, et al Abscisic acid plays an important role in the regulation of strawberry fruit ripening
. [J]. Plant Physiology
, 2011
, 157
(1
): 188
–199
.14.
Jungang
Wang
, Shuzhen
Zhang
, Benpeng
Yang
et al 3,5-dinitrosalicylic acid (DNS) method was used to determine the content of total sugar and reducing sugar in sugarcane stem node
[J]. Sugarcane and Canesugar
, 2008
(5
): 45
–49
.15.
Weiwei
Han
, Ning
Han
, Kaikai
Jing
et al Effect of film-forming anti-transpiration agent on ripening process of cabernet sauvignon
[J]. Acta Horticulturae Sinica
, 2018
(3
).16.
Yubo
Hua
. Effects of plastic treatment on cabernet sauvignon disease and fruit quality
[D]. Yanglin
: Northwest A&F University
, 2010
.17.
Fu
J.
, Chu
J.
, Sun
X.
, et al Simple, rapid, and simultaneous assay of multiple carboxyl containing phytohormones in wounded tomatoes by UPLC-MS/MS using single SPE purification and isotope dilution
.[J]. Analytical Sciences
, 2012
, 28
(11
): 1081
–1087
.18.
Qingjie
Wang
, Zhongxin
Jin
, Lijie
Zhou
et al Effects of overexpression of MdcyMDH in apples on photosynthesis, hormones and growth
[J]. Scientia Agricultura Sinica
, 2015
, 48
(14
): 2868
–2875
.19.
Xinglong
Ji
, Ning
Han
, Heng
Zai
et al Screening of extender protein gene in response to the infection of grape phylloxera and preparation of polyclonal antibodies
[J]. Acta Horticulturae Sinica
, 2016
, 43
(1
): 15
–24
20.
S. D.
Castellarin
, G.
Digaspero
. Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines
[J]. BMC Plant Biology.
2007
(7
): 46
.21.
K. J.
Livak
, T. D.
Schmittgen
. Analysis of relative gene expression data using real-time quantitative PCR and the the 2-ΔΔCT method
[J]. Method
, 2001
(25
): 402
–408
.22.
Peian
Zhang
, Zhuangwei
Wang
, Binhua
Cai
et al The effect of ABA on the expression of key genes in postharvest ripening of jufeng grape
[J]. Acta Horticulturae Sinica
, 2018
(6
).23.
Peng
Cao
. Study on the characteristics of glucose metabolism related enzymes in grape fruits before and after color transformation [D]
. Yanglin
: Northwest A&F University
, 2004
.24.
Hong
Sun
, Qianyu
Yue
, Guangqing
Xiang
, et al Effects of different concentrations of NaCl on grape fruit quality
[J]. Plant Physiology Journal
, 2018
(1
): 63
–70
.25.
S. D.
Castellarin
, A.
Pfeiffer
, P.
Sivilotti
, et al Transcriptional regulation of anthocyanin biosynthesis in ripening fruits of grapevine under seasonal water deficit
[J]. Plant Cell & Environment
, 2010
, 30
(11
): 1381
–1399
.26.
Zhang
M.
, Leng
P.
, Zhang
G.
, et al Cloning and functional analysis of 9-cis-epoxycarotenoid dioxygenase (NCED) genes encoding a key enzyme during abscisic acid biosynthesis from peach and grape fruits
. [J]. Journal of Plant Physiology
, 2009
, 166
(12
): 1241
–1252
.27.
L. G.
Deluc
, J.
Grimplet
, M. D.
Wheatley
, et al Transcriptomic and metabolite analyses of cabernet sauvignon grape berry development
. Bmc Genomics
, 2007
, 8
(1
), 429
–429
.28.
Liang
S.
, Mei
Z.
, Jie
R.
, et al Reciprocity between abscisic acid and ethylene at the onset of berry ripening and after harvest
[J]. Bmc Plant Biology
, 2010
, 10
(1
): 1
–11
.
This content is only available via PDF.
© 2020 Author(s).
2020
Author(s)
You do not currently have access to this content.
