Basal stem rot (BSR) disease is a devastating threat to Malaysia oil palm plantation, which is caused by Ganoderma boninense fungi. Dikaryon is the dominant stage of G. boninense and the pathogenic form of the fungus as compared to the monokaryon. Mating signalling response lead by mitogen-activated pathway (MAPK) responsible in the transformation of sterile monokaryon to pathogenic dikaryon. Expression of the STE3 gene involved in the autocrine activation of mating pathway. Mutations of STE3 gene in some pathogenic yeast resulted in inhibition of pheromone responses and blocked the signalling in the cell fusion. Identification of pheromone receptor, STE3 gene involved in mating signalling pathway is important to understand the mating response towards pathogenesis of G. boninense. Genome walking and polymerase chain reaction (PCR) were carried out to amplify isolate the full length of STE3 gene. Several in silico sequence analysis were conducted to characterise the STE3 gene. The full-length gene sequence including the upstream and downstream regions obtained could be used in future studies to understand pathogenesis of G. boninense.
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27 June 2019
THE 2018 UKM FST POSTGRADUATE COLLOQUIUM: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium
4–6 April 2018
Selangor, Malaysia
Research Article|
June 27 2019
Identification and characterization of a mating signalling gene from an oil palm pathogen, Ganoderma boninense Available to Purchase
Ahmad Zairun Madihah;
Ahmad Zairun Madihah
a)
1
School of Biosciences and Biotechnology, Faculty Science and Technology, Universiti Kebangsaan Malaysia
, 43600 Bangi, Selangor, Malaysia
2
Ganoderma and Diseases Research for Oil Palm (GanoDROP) Unit, Biology Research Division, Malaysian Palm Oil Board (MPOB),6, Persiaran Institusi
, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia
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Abu Seman Idris;
Abu Seman Idris
b)
2
Ganoderma and Diseases Research for Oil Palm (GanoDROP) Unit, Biology Research Division, Malaysian Palm Oil Board (MPOB),6, Persiaran Institusi
, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia
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Shazilah Kamaruddin;
Shazilah Kamaruddin
c)
1
School of Biosciences and Biotechnology, Faculty Science and Technology, Universiti Kebangsaan Malaysia
, 43600 Bangi, Selangor, Malaysia
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Izwan Bharudin;
Izwan Bharudin
d)
1
School of Biosciences and Biotechnology, Faculty Science and Technology, Universiti Kebangsaan Malaysia
, 43600 Bangi, Selangor, Malaysia
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Farah Diba Abu Bakar;
Farah Diba Abu Bakar
e)
1
School of Biosciences and Biotechnology, Faculty Science and Technology, Universiti Kebangsaan Malaysia
, 43600 Bangi, Selangor, Malaysia
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Abdul Munir Abd Murad
Abdul Munir Abd Murad
f)
1
School of Biosciences and Biotechnology, Faculty Science and Technology, Universiti Kebangsaan Malaysia
, 43600 Bangi, Selangor, Malaysia
f)Corresponding author: [email protected]
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Ahmad Zairun Madihah
1,2,a)
Abu Seman Idris
2,b)
Shazilah Kamaruddin
1,c)
Izwan Bharudin
1,d)
Farah Diba Abu Bakar
1,e)
Abdul Munir Abd Murad
1,f)
1
School of Biosciences and Biotechnology, Faculty Science and Technology, Universiti Kebangsaan Malaysia
, 43600 Bangi, Selangor, Malaysia
2
Ganoderma and Diseases Research for Oil Palm (GanoDROP) Unit, Biology Research Division, Malaysian Palm Oil Board (MPOB),6, Persiaran Institusi
, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia
f)Corresponding author: [email protected]
AIP Conf. Proc. 2111, 040005 (2019)
Citation
Ahmad Zairun Madihah, Abu Seman Idris, Shazilah Kamaruddin, Izwan Bharudin, Farah Diba Abu Bakar, Abdul Munir Abd Murad; Identification and characterization of a mating signalling gene from an oil palm pathogen, Ganoderma boninense. AIP Conf. Proc. 27 June 2019; 2111 (1): 040005. https://doi.org/10.1063/1.5111244
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