A cellulase-producing bacterium, designated Q-3, was isolated from silage corn on CMC-Na Congo Red medium plates. It was further identified as Bacillus subtilis using morphological investigation and 16S rDNA sequencing analysis. The cellulase-producing fermentation conditions of the Q-3 isolate were optimized by single factor experiment and orthogonal test. The results showed the optimal cellulase-producing conditions of the Q-3 strain were grown at 37 °C for 72 h with the shaking speed of 180 rpm in a liquid medium containing 2% sodium carboxymethyl cellulose (CMC-Na) with pH 5.4. Under the optimal conditions, the maximum cellulase activity of the Q3 bacterial supernatant was 18.667 U/mL.

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