A cellulase-producing bacterium, designated Q-3, was isolated from silage corn on CMC-Na Congo Red medium plates. It was further identified as Bacillus subtilis using morphological investigation and 16S rDNA sequencing analysis. The cellulase-producing fermentation conditions of the Q-3 isolate were optimized by single factor experiment and orthogonal test. The results showed the optimal cellulase-producing conditions of the Q-3 strain were grown at 37 °C for 72 h with the shaking speed of 180 rpm in a liquid medium containing 2% sodium carboxymethyl cellulose (CMC-Na) with pH 5.4. Under the optimal conditions, the maximum cellulase activity of the Q3 bacterial supernatant was 18.667 U/mL.
REFERENCES
1.
Tabbassum
K
, Wajeeha
A
, Munazza
A
, et al. Industrially relevant cellulase production by indigenous thermophilic Bacillus licheniformis TLW-3 strain: Isolation-molecular identification and enzyme yield optimization
, Pakistan Journal of Pharmaceutical Sciences
, 2018
, 31
(6
):2333
–2340
.2.
Meenu
K
, Singh
G
, Vishwakarma
R A.
Chapter 22-molecular mechanism of cellulose production systems in Trichoderma. Biotechnology and Biology of Trichoderma
, Gupta
V K
, Schmoll
M
, Herrera-estrella
A
, et al, Amsterdam
: 2014
, 319
–324
.3.
Andersen
N.
Enzymatic hydrolysis of cellulose. Copenhagen
: 2007
.4.
Lan
S L
, Chen
X.
Li
H.
et al. Selection of the cellulose producing strain TP1202 and the study on the conditions for cellulose production
. Biotechnology
, 2003
, 13
(2
): 12
–13
.5.
Xie
Z L
, Wu
R
, Li
X P.
et al. Screening of a high cellulose producing strain on the Qinghai plateau
. Chinese Journal of Veterinary Science and Technology
, 2003
, 33
(8
): 50
–53
.6.
Kuhad
R C
, Gupta
R
, Singh
A.
Microbial cellulases and their industrial applications
. Enzyme Research
, 2011
: 1
–10
.7.
Gupta
R
, Gigras
P
, Mohapatra
H.
et al. Microbial α-amylases: a biotechnological perspective
. Process Biochemistry
, 2003
, 38
(11
): 1599
–1616
.8.
Chen
B
, Lin
K J.
Studies on alkaline cellulase produced by Bacillus sp No
.. Acta Agricultural Zhejiangenses
, 1994
, 6
(1
): 37
–40
.9.
Tian
X Y
, Wang
X.
Production and some properties of alkaline cellulose
. Microbiology
, 1997
, 24
(4
): 195
–198
.10.
Wang
W
, Fu
J H
, Gui
W D.
et al. Studies on the cultural condition of strain Bacillus subtilis cellulose
. Xinjiang Agricultural Sciences
, 2000
, 18
(4
): 20
–22
.11.
Kasana
R C
, Salwan
R
, Dhar
H.
et al. A rapid and easy method for the detection of cellulases on agar plates using grams iodine
. Current Microbiology
, 2008
, 57
(5
): 503
–507
.12.
Zhu
LP
. Isolation and molecular identification of Lactobacillus brevis from spoilage craft beer in China, Advances in Applied Biotechnology
, Lecture Notes in Electrical Enginee
, 2018
, 444
,597
–603
.13.
Shen
X L
, Xia
L M.
Studies on cellulose production by Bacillus sp ZU-04
. Chemistry and Industry of Forest Products
, 2002
, 22
(3
): 54
–58
.14.
Song
G J
, Wang
D
, Sun
C Y
, et al. Studies on the alkaline cellulose of the Bacillus sp. 074 I-Isolation selection and determination of the ferment conditions of the strain
. Acta Microbiologica Sinica
, 1995
, 35
(1
): 38
–44
.15.
Cherry
J R
, Fidanisef
A L.
Directed evolution of industrial enzymes: an update
. Curr Opin Biotechnol
, 2003
, 14
: 438
–443
.16.
Lee
Y
, Kim
B
, Lee
B
, et al. Purification and characterization of cellulose produced by bacillus amyoliquefaciens dl-3utilizing rice hull
. Bioresource Technology
, 2008
, 99
(2
): 378
–386
.
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