Gingivitis may be caused by endogenous and exogenous factors. One of the exogenous factors is caused by chemical compounds such as 35% H2O2, a dental bleaching agent. Dental bleaching is a conservative measure to improve tooth color. This agent may cause inflammation of the mucosa around the teeth like the gingiva. The gingiva may become hyperemic, causing pain and changes in the gingival contour. Treatment of gingivitis basically suppresses inflammatory reactions and speeds the healing process. Histologically, inflammation healing shows an increase in the number of fibroblasts. This study was aimed to investigate the effect of 50% propolis ethanol extract (EEP) in 35% H2O2−induced male Wistar rats. This research was conducted with an experimental design (Randomized posttest only with control group design), consisting of 2 groups, namely the control group and the treatment group. The control group was given aquadest and the treatment group was given propolis ethanol extract with a concentration of 50% topically. The results were analyzed statistically using computer. Mann Whitney U Test showed a significantly higher number of fibroblasts in the treatment group compared to the control group (p<0.05). It can be concluded that the administration of 50% EEP could increase the number of fibroblasts in the healing process of gingival inflammation in 35% H2O2−induced rats.

Topics
Educational assessment, Inflammation
1.
Carranza
 
F.A
,
Takei
 
H
,
Newman
 
MG
. The periodontal disease. In:
Carranza
 
F.A
,
Forres
 
JL
,
Kenney
 
EB
,
Klokkevold
 
PR
,
Newman
 
MG
,
Novack
 
MJ
,
Preshaw
 
P
,
Takei
 
HH.
 
Caranza’s Clinical Periodontology
. 12th ed 
St. Louis
:
Saunders Elsevier
.
2014
.
Google Scholar
 
2.
Nanci
 
A
,
Martha
 
JS
 The Cate”S:Oral Histology Development,
Stucture and Function
. 8th.
St. Louis
:
Mosby
.
2008
:
297
–
8
Google Scholar
 
3.
Matis
 
BA
,
Cohran
 
MA
,
Franco
 
M
,
Al-Ammar
 
W
,
Eckert
 
GJ
,
Stropes M.
 
Eight
 
IN-Office Toothwhitening System Evaluated
In
Vivo: apilot study. Rvista Romana DeStomatologie
,
2007
:
3
(
3
):
131
–
7
Google Scholar
 
4.
Dumitrescu
 
AL
,
Kobayashi
 
J
: Genetic Variants In Periodontal Health and Disease.
Springer Dordrecht
 
Heidelberg London-New York
.
2010
:
1
–
9
5.
Li
 
Y
,
Greenwall
.
Safety issues of tooth whitening using peroxide-based materials
.
British Dental Journal.
 
2013
:
5
(
1
):
29
–
33
https://doi.org/10.1038/sj.bdj.2013.629
Google Scholar
Crossref
Search ADS
 
6.
Bognadov
 
S
:
Propolis: Composition, Health, Medical
.
Bee Product Science
, www.bee-hexagon.net. February.
2014
.
Google Scholar
 
7.
Bloom
,
Fawcet
,
W.D
:
ATextbook of Hostology.
 12th Ed 
New York
:
Chapman and Hall
.
2002
:
133
–
167
Google Scholar
 
8.
Sabir
 
A
:
Respon Inflamasi Pada Pulpa Gigi Tikus setelah Aplikasi Ekstrak Etanol Prpolis
:
Maj. Ked Gigi (Dent J)
.
2005
.a:
38
:
2
:
77
–
83
https://doi.org/10.20473/j.djmkg.v38.i2.p77-83
Google Scholar
Crossref
Search ADS
 
9.
Puspasari
 
A
,
Harijanti
 
K
,
Soebadi
 
B
,
Hendarti
 
H.T
,
Radithia
 
D
,
Ernawati
 
D.S
:
Effects Of Topical Aplication Of Propolis Extract On Fibroblast Growth Factor-2 and Fibroblast Expression In The Traumatic Ulcer Of Diabetic Rattus Norvegicus
,
2018
:
J. Of Oral and Maxillofacial Pathology
:
22
:
1
:p.
54
–
58
Google Scholar
 
10.
Najeh
 
S
,
Ali
 
A.H
:
In Vitro toxicity Of Propolis In Comparison Eith Other Primary Teeth Pulpotomy Agents On Human Fibroblast
:
J Of Investigative and Clinical Dentistry
:
2016
:
7
:
308
–
313
https://doi.org/10.1111/jicd.12157
Google Scholar
Crossref
Search ADS
 
11.
Kartika
 
D.C
,
Kunarti
 
S
,
Subiyanto
 
A
:
Genotoxicity Test Of Propolis Extract, Mineral Trioksida Agregat, And Calcium Hydroxide On Fibroblast BHK-21 Cell Cultures
,
2015
:
Dent. J.
:
48
:
1
:p
16
–
21
Google Scholar
Crossref
Search ADS
 
12.
Suranto
 
A
 dr. Sp A:
Dahsyatnya Propolis Untuk Menggempur Penyakit
,
Agromedia Pustaka Jakarta
:
2010
13.
Novilla
 
A
:
Aktifitas Antioksidan Ekstrak Propolis Lebah Madu Apis Melifera
,
J. Kesh Kartika
,
2006
:p
64
–
72
Google Scholar
 
14.
Sabir
 
A
,
Impact Of The Use Of Ethanolic Extract Of Propolis, Flavonoid and Non Flavonoid Propolis For Direct Pulp Capping In Collagen Type I Density
.
Braz J. Oral Sci
:
2016
:
15
:
4
:
264
–
268
https://doi.org/10.20396/bjos.v15i4.8650037
Google Scholar
Crossref
Search ADS
 
15.
Nanci
 
A
,
Martha
 
JS
:
The Cate’s Oral Histology Development, Structure And Function
. In
Clinical Periodontolog
, 10th ed,
2010
Google Scholar
 
16.
Moraes
 
L
,
Trevilatto
,
P
,
Gregio
 
A
,
Machado
 
M
,
Lima
 
A
:
Quantitative Analysis Of Mature and Immature Collagens During Oral Wound Healing In Rats Treated by Brazillian Propolis
,
J. of International Dental and Medical Research
,
2011
:
3
:p.
106
–
110
Google Scholar
 
17.
Tyszka
 
M
,
Czohara
,
Pasko
 
P
:
Zinc AND Propolis Reduce Cytoxicity and Proliferation in Skin Fibroblast Cell Culture: Total Polyphenol Content and Antioxidant Capacity of Propolis
.
Biol Trace Elem Res
,
2014
:
160
:
123
–
131
https://doi.org/10.1007/s12011-014-0019-3
Google Scholar
Crossref
Search ADS
PubMed
 
18.
Jungueira
 
L.C.
 dan
Corneiro
:
Histologi Dasar Teks dan Atlas. Alih Bahasa dr. Jan Tambayong
. Editors
Frans
 
Danny.
 10thed,
Jakarta
,
2007
:
EGC Jakarta
: p
220
–
230
Google Scholar
 
19.
Subowo
,
Histologi Umum, ED 2
,
Sagung Seto
:
Jakarta
:
2009
20.
Putri
 
VE
,
Adi
 
P.
,
Fidya
,
Pegaruh Pemberian Propolis Lebah Madu Apis Mellifera Terhadap Jumlah Makrofag Pada Pulpa Terbuka
,
Malang
:
2011
Google Scholar
 
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