Perspectives on cell therapy using multipotent stem cells or progenitor stem cells are progressing rapidly. Therefore, biomedical research on cells detection method should in line with it. Flow cytometry technique is usually used quantitatively to see the presence of stem cells in different sources, such as blood, bone marrow, tissues, etc. Nevertheless, there is another modality that can be used as an alternative, i.e. polymerase chain reaction (PCR). This study aimed to know whether PCR could be used as an alternative method for replacing flow cytometry technique in detecting human mononuclear cells (hMNCs) derived from umbilical cord blood in the rat blood. Human mononuclear cells from umbilical cord blood were injected into rat tail’s vein. Blood sampling was collected from the heart 30 minutes, 1 hour, 3 hours, 6 hours, 1 day and 7 days after injection. The blood was then analyzed with flow cytometry to determine the presence of hMNCs (CD34+ hMNCs) and PCR to determine the presence of human DNA (human chromosome 17 alpha satellite DNA; HSA17). Polymerase chain reaction could be detecting the presence of human DNA in all samples that are compared to the flow cytometry that also detect the presence of hMNCs in all samples. The results showed that flow cytometry and PCR technique have a similar trend pattern based on time dependent. Polymerase chain reaction techniques can be used as an alternative method in detecting the presence of hMNCs in the rat’s blood equally to the flow cytometry.
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9 April 2019
3RD BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, AND MEDICAL DEVICES : Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2018
6–8 August 2018
Jakarta, Indonesia
Research Article|
April 09 2019
Comparison study of human mononuclear cells presence in rat blood using flow cytometry and polymerase chain reaction
Iqbal Fasha;
Iqbal Fasha
1
Department of Medical Biology, Faculty of Medicine, Universitas Indonesia
, Jl. Salemba Raya No. 6, Central Jakarta 10430 Indonesia
2
Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia
, Jl. Salemba Raya No. 6, Central Jakarta 10430 Indonesia
3
Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo Central Hospital-Faculty of Medicine, Universitas Indonesia
, Jl. Salemba Raya No. 6, Central Jakarta 10430 Indonesia
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Isabella Kurnia Liem;
Isabella Kurnia Liem
3
Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo Central Hospital-Faculty of Medicine, Universitas Indonesia
, Jl. Salemba Raya No. 6, Central Jakarta 10430 Indonesia
4
Department of Anatomy, Faculty of Medicine, Universitas Indonesia
, Jl. Salemba Raya No. 6, Central Jakarta 10430 Indonesia
5
Integrated Laboratory, Faculty of Medicine, Universitas Indonesia
, Jl. Salemba Raya No. 6, Central Jakarta 10430 Indonesia
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Radiana D. Antarianto;
Radiana D. Antarianto
6
Department of Histology, Faculty of Medicine Universitas, Indonesia
, Jl. Salemba Raya No. 6, Central Jakarta 10430 Indonesia
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Imelda Rosalyn Sianipar;
Imelda Rosalyn Sianipar
a)
7
Department of Medical Physiology, Faculty of Medicine, Universitas Indonesia
, Jl. Salemba Raya No. 6, Central Jakarta 10430 Indonesia
a)Corresponding author: [email protected]
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Luluk Yunaini
Luluk Yunaini
b)
1
Department of Medical Biology, Faculty of Medicine, Universitas Indonesia
, Jl. Salemba Raya No. 6, Central Jakarta 10430 Indonesia
2
Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia
, Jl. Salemba Raya No. 6, Central Jakarta 10430 Indonesia
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a)Corresponding author: [email protected]
AIP Conf. Proc. 2092, 030010 (2019)
Citation
Iqbal Fasha, Isabella Kurnia Liem, Radiana D. Antarianto, Imelda Rosalyn Sianipar, Luluk Yunaini; Comparison study of human mononuclear cells presence in rat blood using flow cytometry and polymerase chain reaction. AIP Conf. Proc. 9 April 2019; 2092 (1): 030010. https://doi.org/10.1063/1.5096714
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