Many studies indicate a crucial role of vitamin B12 in hyperhomocysteinemia and hyperglicemia. In this study, through dietary restriction, we developed rat models of vitamin B12 deficiencies to elucidate the impact on hyperhomocysteinemia and hyperglycemia. We investigated whether there is a correlation between plasma Vitamin B12 with homocysteine and the presence of hyperglycemia in male rats. Adult male mice were divided into 3 groups (a) Control diet for 16 weeks (b) vitamin B12-restricted diet with pectin as dietary fiber for 8 weeks (K1) (c) vitamin B12-restricted diet with pectin as dietary fiber for 16 weeks (K2). After 8 weeks of feeding, plasma concentrations of vitamin B12, homocysteine, and glucose were assessed. Blood samples for vitamin B12, homocysteine and glucose were obtained after overnight fasting. After 16 weeks of continued feeding on their respective diets, plasma concentrations of vitamin B12, homocysteine, and glucose were assessed. Plasma vitamin B12 levels were lower in K2 compared to both the control and K1 groups. On the other hand, the deficient B12 group had significantly higher levels of homocysteine and glucose than control on 8 and 16 weeks. In this study, we concluded that hyperglycemia was associated with lower serum vitamin B12 concentration and serum homocysteine in the development of vitamin B12 deficient rat model.
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9 April 2019
3RD BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, AND MEDICAL DEVICES : Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2018
6–8 August 2018
Jakarta, Indonesia
Research Article|
April 09 2019
Developing vitamin B12 deficient rat model based on duration of restriction diet: Assessment of plasma vitamin B12, homocysteine (Hcy), and blood glucose levels
Imelda Rosalyn Sianipar;
Imelda Rosalyn Sianipar
a)
1
Department of Physiology, Faculty of Medicine, Universitas Indonesia
, Indonesia
a)Corresponding author: [email protected]
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Irena Ujianti;
Irena Ujianti
2
Master Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia
, Indonesia
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Sophie Yolanda;
Sophie Yolanda
1
Department of Physiology, Faculty of Medicine, Universitas Indonesia
, Indonesia
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Aditya K. Murthi;
Aditya K. Murthi
2
Master Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia
, Indonesia
3
Department of Physiology, Faculty of Medicine, Trisakti University
, Indonesia
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Patwa Amani;
Patwa Amani
3
Department of Physiology, Faculty of Medicine, Trisakti University
, Indonesia
4
Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia
, Indonesia
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Dewi Irawati Soeria Santoso
Dewi Irawati Soeria Santoso
1
Department of Physiology, Faculty of Medicine, Universitas Indonesia
, Indonesia
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a)Corresponding author: [email protected]
AIP Conf. Proc. 2092, 020004 (2019)
Citation
Imelda Rosalyn Sianipar, Irena Ujianti, Sophie Yolanda, Aditya K. Murthi, Patwa Amani, Dewi Irawati Soeria Santoso; Developing vitamin B12 deficient rat model based on duration of restriction diet: Assessment of plasma vitamin B12, homocysteine (Hcy), and blood glucose levels. AIP Conf. Proc. 9 April 2019; 2092 (1): 020004. https://doi.org/10.1063/1.5096672
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